哮喘患者血清IL-13、TGF-β1、VEGF表达变化及与气道炎症、重塑的关系探讨

Changes in expressions of serum IL-13，TGF-β1，VEGF and their relationship with airway inflammation and remodeling in patients with asthma

摘要:

目的：本研究从细胞因子角度出发探讨白介素-13（Interleukin-13，IL-13）、转化生子因子-β1（Transforming growth factor-β1，TGF-β1）、血管内皮生子因子（Vascular endothelial growth factor，VEGF）血清浓度是否在哮喘患者的气道慢性炎症及重塑的发病机制中起着调控作用及其可能途径。方法：选择哮喘急性发作期患者36例（中重度发作20例、轻度发作16例）、缓解期36例、健康体检者36例。取肘静脉血，用ELISA方法测定血清中IL-13、TGF-β1、VEGF水平；所有入试者均作肺功能检查，并对结果进行统计学分析。结果：血清IL-13、TGF-β1、VEGF的表达量在哮喘患者中存在显著的正相关性；同时三者血清浓度越高，肺功能检查指标第1 s用力呼气容积（Forced expiratory volume in the 1st s，FEV1）、第1 s用力呼气容积占用力肺活量的比值（Ratio of forced expiratory volume in 1 s to forced vital capacity，FEV1/FVC%）越小，提示哮喘的病情程度越重，血清IL-13、TGF-β1、VEGF的表达量与FEV1、FEV1/FVC%呈负相关性。结论：IL-13、TGF-β1、VEGF网络失衡是哮喘发病的主要机制之一，三者在哮喘发病中相互影响，相互促进，共同参与哮喘的气道炎症与重塑的过程；联合检测血清IL-13、TGF-β1、VEGF水平对判断哮喘患者病情严重程度及预后、辅助诊断及治疗具有重要价值。

Abstract:

Objective：To discuss whether serum interleukin-13（IL-13），transforming growth factor-β1（TGF-β1），vascular endothe－lial growth factor（VEGF） play a regulatory role in the pathogenesis of chronic air way inflammation and remodeling and to explore its possible way from the perspectives of cytokine. Methods：Totally 36 cases of acute asthma at oneset stage（20 cases of moderate to severe attack and 16 cases of mild attack），36 cases in remission and 36 healthy cases were enrolled. The venous blood was extracted at the elbow. ELISA was used to determine serum IL-13，TGF-β1 and VEGF level；lung function testing was performed on all patients and the results were statistically analyzed. Results：The expressions of serum IL-13，TGF-β1 and VEGF were positively correlated in patients with asthma. At the same time，the higher the serum concentrations of the above three indicators，the lower the forced expiratory volume in the 1st s（FEV1） and the ratio of FEV1 to forced vital capacity（FEV1/FVC%），indicating that asthma becoming more and more serious. The expressions of serum IL-13，TGF-β1 and VEGF were negatively correlated with FEV1 and FEV1/FVC%. Conclu－sions：The network imbalance of IL-13，TGF-β1 and VEGF is one of the main mechanisms for asthma onset. Mutual influence and promotion are found among the three indicators in chronic air way inflammation and remodeling. United detection of serum IL-13，TGF-β1 and VEGF is of great importance in judging disease severity and providing prognosis，auxiliary diagnosis and treatment for patients with asthma.