PTP4A1在舌鳞癌组织及TCA8113细胞中的表达及意义

Expressions of PTP4A1 in TCA8113 cell line and tongue squamous cell carcinoma tissues and their clinical significances

摘要:

目的：检测PTP4A1基因在舌鳞癌组织及舌鳞癌细胞系TCA8113中的表达，并探讨其表达水平与舌鳞癌临床病理参数的关系。方法：应用免疫组化SP法检测30例舌鳞癌和15例正常舌组织石蜡包埋组织中PTP4A1的表达；对15例新鲜舌鳞癌组织进行real-time PCR，检测PTP4A1在舌鳞癌组织、癌旁组织、正常舌组织的表达，此外对TCA8113细胞中PTP4A1的表达水平也进行了检测。结果：免疫组化结果显示，PTP4A1表达于舌鳞癌组织的细胞质和细胞核，在舌鳞癌组织中呈阳性和强阳性表达，而在正常舌组织中呈阴性和弱阳性表达；PTP4A1在舌鳞癌的阳性表达率为83%，显著高于其在正常舌组织中的表达率33.3%；PTP4A1在舌鳞癌组织和正常舌组织的表达差异有统计学意义（P=0.001<0.05）；而且PTP4A1的表达水平与肿瘤的淋巴转移相关（P=0.014<0.05），而与病人的性别、年龄、肿瘤的大小、肿瘤的分期分级无明显相关。Real-time PCR实验结果显示舌鳞癌组织及舌鳞癌细胞系TCA8113细胞中的PTP4A1的mRNA表达水平高于其在癌旁和正常舌组织的表达，癌旁组织的表达高于正常舌组织的表达（P=0.000<0.05）。结论：PTP4A1基因mRNA水平和蛋白水平表达在舌鳞癌组织中表达均较癌旁和正常舌组织高，可能在舌鳞癌的发生发展过程中起着一定作用。

Abstract:

Objective：To investigate PTP4A1 gene expressions in human tongue squamous cancer tissues and TCA8113 cell line and to explore the relationship between PTP4A1 expressions and clinicopathological features of human tongue squamous cancer. Methods：Expressions of PTP4A1 in 30 cases of human tongue squamous cell carcinoma and 15 cases of normal tongue mucosa paraffin-embed－ded specimens were examined by immunohistochemical technology and in 15 freshly-taken tongue squamous tissues and TCA8113 cells by real-time PCR. Results：Immunoreactive staining was located in the cytoplasm and cell nucleus of human tongue squamous cancer. Positive rate of PTP4A1 in human tongue squamous cancer was 83%，which was remarkably higher than that in normal tongue tissue 33.3%（P=0.001<0.05），with significant differences. Expression of PTP4A1 was closely related with lymphatic metastases（P=0.014<0.05），but had no relationship with age，gender，tumor size，and clinical stage. Result of real-time PCR also showed that PTP4A1 mRNA level was higher in human tongue squamous cancer tissues and TCA8113 cells than in para-carcinoma and normal tissues（P=0.000<0.05）. Conclusions：Both PTP4A1 mRNA and protein expressions are higher in human tongue squamous cancer tissues than in para-carcinoma and normal tongue tissues. PTP4A1 expression may play a significant role in human tongue squamous cancer develop－ment and metastases.