顺铂联合紫杉醇分次给药抑制卵巢癌细胞SKOV3的种群恢复

Dose-dense administration of combinative paclitaxel and cisplatin might suppress the repopulation of ovarian cancer cell SKOV3

摘要:

目的：比较顺铂（cisplatin，CDDP）与紫杉醇（paclitaxel，PTX）不同的给药方案对卵巢癌细胞种群恢复的影响。方法：（1）CDDP、PTX或CDDP联合PTX分别于第1天（D1）或D1及D8处理卵巢癌SKOV3细胞；（2）倒置显微镜观察不同处理组中，SKOV3细胞凋亡与种群恢复的情况；（3）CCK-8法分别测定不同处理组在D7和D21时SKOV3细胞的吸光度（absorbance，A）值。结果：（1）单用CDDP组中，单次给药组与分次给药组相比较，二者对SKOV3细胞的凋亡与种群恢复的影响无明显差异（P=0.970）；（2）单用PTX组中，与单次给药组相比，分次给药组明显抑制了SKOV3细胞的种群恢复（P=0.000）；（3）CDDP联合PTX组中，分次给药组较单次给药组对SKOV3细胞的种群恢复明显抑制（P=0.000），并且在分次给药组中，CDDP联合PTX组较单用PTX组明显抑制SKOV3细胞的种群恢复（P=0.000）。结论：CDDP联合PTX分次给药较单次给药可能通过抑制卵巢癌细胞的种群恢复从而抑制卵巢癌的耐药或复发。

Abstract:

Objective：To compare the impact of different dosage regimens of cisplatin（CDDP） or/and paclitaxel（PTX） on the repopu－lation of ovarian cancer cells. Methods：（1）SKOV3 cells were treated with CDDP，PTX or CDDP combined with PTX respective－ly，each arm treated with fractional dose regimen（the total dosage was dividedly administration on d1 and d8） or single dose regimen（the total dosage was administration on d1）；（2）The apoptosis and repopulation of SKOV3 cells were observed under microscope；（3）The cell survival on d7 and d21 in each group was detected by CCK-8 method. Results：（1）In CDDP groups，no significant dif－ference was found between the two regimens（P=0.970）；（2）In PTX groups，the repopulation of SKOV3 cells in fractional dose regi－men was significantly inhibited compared with that of single dose regimen（P=0.000）；（3）Fractional schedule significantly inhibited the repopulation of SKOV3 cells in CDDP+PTX groups（P=0.000），and such suppressive efficiency was even more evident in CDDP+PTX group than in PTX monotherapy group（P=0.000）. Conclusion：CDDP+PTX with fractional dose regimen might prevent resistance or recurrence of ovarian cancer cells through significantly inhibiting the repopulation of ovarian cancer cells.