Ghrelin基因在多囊卵巢综合征患者卵巢颗粒细胞中的表达

Expression of Ghrelin gene in ovarian granulosa cells of patients with polycystic ovarian syndrome

摘要:

目的：探讨Ghrelin基因在卵巢颗粒细胞中的表达及其与多囊卵巢综合征（polycystic ovarian syndrome，PCOS）的关系。方法：选择47例在重庆医科大学辅助生育中心接受体外受精-胚胎移植治疗的不孕妇女，其中PCOS组11例，对照（Non-PCOS）组36例，采用real-time PCR及免疫印迹法分别测定2组妇女卵巢颗粒细胞中Ghrelin mRNA和蛋白的表达水平。结果：PCOS妇女颗粒细胞中Ghrelin mRNA和蛋白的表达均低于Non-PCOS妇女（0.926±0.206 vs. 1.137±0.105，t=2.73，P=0.015；0.647±0.052 vs. 0.937±0.038，t=7.82，P=0.001）。结论：PCOS妇女卵巢颗粒细胞的Ghrelin mRNA和蛋白表达水平明显低于Non-PCOS妇女，可能与卵泡发育障碍相关。

Abstract:

Objective：To determine the expression of Ghrelin gene in ovarian granulosa cells（GCs） of patients with polycystic ovarian syndrome（PCOS） and to discuss the relationship between them． Methods：GCs were obtained at the time of oocyte retrieval from a total of 47 women undergoing in vitro fertilization and embryo transfer（IVF-ET） treatment due to male factors or tubal factors. Eleven PCOS women were taken as PCOS group and 36 non-PCOS women as control group. The expression of Ghrelin mRNA was measured by real-time PCR and Western blot was used to detect the expression of Ghrelin protein in GCs. Results：The expression of Ghrelin mRNA in GCs was significantly lower in PCOS group than in control group（t=2.73，P=0.015） and the expression of Ghrelin protein in GCs was also significantly lower in PCOS group than in control group（t=7.82，P=0.001）. Conclusion：The mRNA and protein expressions of Ghrelin in GCs are significantly lower in women with PCOS than in non-PCOS women，suggesting that Ghrelin may be involved in the disturbance of follicular development in PCOS.