长链非编码RNA-LINC00152在甲状腺乳头状癌中的表达及功能研究

Expression and function of long non-coding RNA-LINC00152 in papillary thyroid carcinoma

摘要:

目的：长链非编码RNA-LINC00152在多种类型的恶性肿瘤中发挥致癌作用，但其在甲状腺乳头状癌（papillary thyroid carcinoma，PTC）尚不清楚。方法：纳入32例PTC患者。术中从PTC患者收集肿瘤组织和相邻的正常组织。通过qRT-PCR检测LINC00152在这些组织中的表达。敲降LINC00152，建立LINC00152低表达PTC细胞系，并通过Transwell迁移和侵袭实验、细胞克隆实验、生长实验、划痕实验，研究LINC00152在PTC发生发展中起到的作用。结果：大多数PTC患者肿瘤组织中LINC00152的表达水平高于相邻正常组织（n=64，P<0.01）。体外细胞实验证明，敲降LINC00152使PTC细胞的生长增殖、侵袭迁移等恶性能力降低。结论：长链非编码RNA-LINC00152在PTC中呈显著高表达，降低LINC00152可明显降低PTC细胞的恶性能力。

Abstract:

Objective：To investigate the role of long non-coding RNA LINC00152 in papillary thyroid carcinoma（PTC）. Methods：A total of 32 patients with PTC were enrolled. Tumor tissue and adjacent normal tissue samples were collected during surgery，and qRT-PCR was used to measure the expression of LINC00152 in these samples. Knockdown of LINC00152 was performed to establish a PTC cell line with low expression of LINC00152，and Transwell migration and invasion experiments，cell cloning experiments，growth experiments，and wound healing assay were used to investigate the role of LINC00152 in the development and progression of PTC. Results：In 64 of all patients with PTC，the expression of LINC00152 in tumor tissue was higher than that in adjacent normal tissue（P<0.01）. In vitro cell experiments demonstrated that knockdown of LINC00152 reduced the growth，proliferation，invasion，and mi－gration abilities of PTC cells. Conclusion：Long non-coding RNA-LINC00152 has high expression in PTC and reduction in LINC00152 can significantly reduce the malignant ability of PTC cells.