SOX9蛋白在胃癌组织中的表达及与临床病理特征和预后的关系

Expression of SOX9 in gastric cancer tissues and its relationship with clinicopathological features and prognosis

摘要:

目的：探讨SOX9在胃癌组织中的表达变化及与患者临床病理特征和预后的关系。方法：收集2012年6月到2017年6月来新乡医学院第一附属医院接受治疗的180例胃癌患者临床病例资料。收集患者术后胃癌组织和癌旁组织，采用免疫组化染色检测SOX9蛋白的表达水平，并分析SOX9蛋白水平与胃癌患者临床病理特征、预后和预后因素的关系。结果：免疫组化染色结果显示，SOX9蛋白胃癌组织和癌旁组织中主要表达于细胞核中。180例胃癌组织和癌旁组织中SOX9蛋白表达阳性分别为154例 （85.56%）和34例（18.89%），两者SOX9蛋白表达阳性比较差异具有统计学意义（χ2=160.317，P=0.000）。SOX9蛋白表达与临床分期、肿瘤分化程度、是否淋巴结转移及浸润侵及肌层等指标均相关（χ2=6.868，P=0.009；χ2=9.554，P=0.002；χ2=30.456，P=0.000；χ2=29.738，P=0.000）。SOX9蛋白表达阳性组患者中位无进展生存期和总生存期明显低于表达阴性患者，差异有统计学意义（log-rank=3.193，P=0.015；log-rank=5.109，P=0.009）。Cox比例风险回归模型显示，SOX9蛋白表达水平是影响患者术后总生存期和无进展生存期的独立风险因素（P=0.009，P=0.006）。结论： SOX9蛋白表达水平与胃癌患者临床病理特征和预后密切相关，可作为临床胃癌诊断的辅助标记物。

Abstract:

Objective：To investigate the expression changes of SOX9 in gastric cancer tissues and its relationship with clinicopatho－logical characteristics and prognosis of patients with gastric cancer. Methods：The clinical data of 180 patients with gastric cancer treated in our hospital from June 2014 to June 2017 were collected. The expression of SOX9 protein in the patients’ gastric cancer tissues and adjacent normal tissues were detected using immunohistochemical staining. The relationship between SOX9 protein level and clinicopathological features，prognosis and prognostic factors of patients with gastric cancer was analyzed. Results：Immuno－histochemical staining showed that SOX9 protein was mainly expressed in the nucleus of gastric cancer and adjacent tissues. The ex－pression of SOX9 protein in 180 gastric cancer tissues and adjacent tissues was 154（85.56%） and 34（18.89%），respectively. The difference in SOX9 protein expression between the two groups was statistically significant（ χ2=160.317，P=0.000）. The expression of SOX9 protein was correlated with clinical stage，degree of differentia－tion，lymph node metastasis and invasion of muscular layer（ χ2=6.868，P=0.009； χ2=9.554，P=0.002； χ2=30.456，P=0.000； χ2=29.738，P=0.000）. The median progression-free survival and total survival of patients with positive SOX9 expression were significantly lower than those of patients with negative expres－sion（log-rank=3.193，P=0.015；log-rank=5.109，P=0.009）. In Cox proportional risk regression model，the expression of SOX9 pro－tein was an independent risk factor affecting the overall survival and progression-free survival（P=0.009，P=0.006）. Conclusion：The expression level of SOX9 protein is closely related to the clinicopathological characteristics and prognosis of gastric cancer patients，and can be used as an auxiliary marker in the diagnosis of gastric cancer.