胃癌淋巴结转移与microRNA表达水平的相关性分析

Correlation analysis between lymph node metastasis and microRNA expression in gastric cancer

摘要:

目的：探究胃癌发生淋巴结转移与microRNA表达水平的相关性，寻找胃癌治疗的潜在靶点。方法：下载公共数据库TCGA胃癌患者的临床病理信息及miRNA表达情况，根据病理是否存在淋巴结转移分为淋巴结转移阳性组和淋巴结转移阴性组。连续变量资料采用独立样本t检验评估2组之间是否存在差异，分类变量资料采用卡方检验评估2组间是否存在差异，将单因素分析P≤0.01的研究指标纳入logistic多因素回归分析。结果：单因素分析提示71个miRNA的表达水平在2组间差异有统计学意义，2组间肿瘤的T分期和肿瘤的分化程度存在差异。Logistic多因素回归分析显示：hsa-mir-3940（OR=0.713，95%CI=0.524~0.970），hsa-mir-495（OR=1.479，95%CI=1.115~1.963），hsa-mir-580（OR=0.646，95%CI=0.441~0.946），hsa-mir-6720（OR=1.366，95%CI=1.018~1.834），hsa-mir-935（OR=0.862，95%CI=0.747~0.994），T分期（OR=2.831，95%CI=1.728~4.639）。结论：胃癌组织中hsa-mir-3940、hsa-mir-495、hsa-mir-580、hsa-mir-6720、hsa-mir-935的表达及T分期是影响患者淋巴结转移的独立影响因素。

Abstract:

Objective：To investigate the correlation between lymph node metastasis and microRNA expression in gastric cancer，so as to find the potential target of gastric cancer treatment. Methods：The clinical pathological information and miRNA expression of gastric cancer patients from the public database TCGA were downloaded. Patients were divided into the lymph node metastasis positive group and the lymph node metastasis negative group in accordance with the lymph node metastasis. Independent sample t-test was used to evaluate the continuous variable data of the two groups，chi square test was used to evaluate the difference between the two groups，and factors with single factor analysis P≤0.01 were included into the logistic multiple factor regression analysis. Results：Single factor analysis indicated that the expression level of 71 miRNAs had statistically significant difference between the two groups，and tumor T stage and tumor differentiation had significant difference between the two groups. Logistic regression analysis showed that：hsa-mir-3940（OR=0.713，95%CI=0.524-0.970），hsa-mir-495（OR=1.479，95%CI=1.115-1.963），hsa-mir-580（OR=0.646，95%CI=0.441-0.946），hsa-mir-6720（OR=1.366，95%CI=1.018-1.834），hsa-mir-935（OR=0.862，95%CI=0.747-0.994），T stage（OR=2.831，95%CI=1.728-4.639）. Conclusion：The expression of hsa-mir-3940，hsa-mir-495，hsa-mir-580，hsa-mir-6720，hsa-mir-935 and T stage in the gastric cancer tissue are independent influencing factors of lymph node metastasis.