肝细胞癌中microRNA的表达水平与早期复发的相关性分析

Correlation between microRNA expression and early recurrence of hepatocellular carcinoma

摘要:

目的：探讨肝细胞癌组织中microRNA（miRNA）的表达水平与肝细胞癌早期复发的相关性。方法：下载公共数据库TCGA的肝细胞癌患者临床信息、随访结果及miRNA表达情况，根据复发时间将患者分为早期复发组（≤2年复发）和晚期复发组（＞2年复发），连续变量资料采用独立样本t检验评估2组之间是否存在差异，分类变量资料采用卡方检验评估2组间是否存在差异，将单因素分析P≤0.01的指标纳入logistic多因素回归分析。结果：独立样本t检验分析显示，P≤0.01的miRNA分别为hsa-mir-550a-1、hsa-mir-550a-2、hsa-mir-550a-3、hsa-mir-331、hsa-mir-514a-1、hsa-mir-210、hsa-mir-155、hsa-mir-3170、hsa-mir-4677、hsa-mir-4677、hsa-mir-508、hsa-mir-514a-3、hsa-mir-514a-2、hsa-mir-624，卡方检验显示P≤0.01的指标为患者肿瘤T分期。Logistic多因素回归分析显示hsa-mir-155（OR=0.660，95%CI=0.519~0.841）、hsa-mir-331（OR=1.475，95%CI=1.004~2.168）、hsa-mir-514a-1（OR=0.603，95%CI=0.413~0.879）、hsa-mir-550a-1（OR=1.680，95%CI=1.034~2.703）、肿瘤T分期（OR=1.640，95%CI=1.126~2.389）是早期复发的独立影响因素，差异有统计学意义（P＜0.05）。结论：肝细胞癌组织中hsa-mir-155、hsa-mir-331、hsa-mir-514a-1、hsa-mir-550a-1的表达水平和肿瘤T分期是影响肝细胞癌术后早期复发的独立影响因素。

Abstract:

Objective：To investigate the correlation between expression of microRNA（mRNA） and early recurrence of hepatocellular carcinoma（HCC）. Methods：The clinical information，follow-up results and the expression of miRNAs in TCGA were downloaded from the public database. According to the recurrence time，patients were divided into the early-stage recurrence group（≤2 years recur－rence） and the late-stage recurrence group（>2 years recurrence）. Independent sample t test was used to assess the difference of con－tinuous variable data in two groups，and chi-square test was used to evaluate the difference of categorical variable data in two groups. Factors（P<0.01） was included in logistic multivariate regression analysis. Results：T-test analysis showed that the miRNA（P≤0.01） was hsa-mir-550a-1，hsa-mir-550a-2，hsa-mir-550a-3，hsa-mir-331，hsa-mir-514a-1，hsa-mir-210，hsa-mir-155，hsa-mir-mir-3170，hsa-mir-4677，hsa-mir-4677，hsa-mir-508，hsa-mir-514a-3，hsa-mir-514a-2 and hsa-mir-624. The chi-square test showed that the indicator（P≤0.01） was tumor T stage. Logistic multivariate regression analysis showed that hsa-mir-155（OR=0.660，95%CI=0.519-0.841），hsa-mir-331（OR=1.475，95%CI=1.004-2.168），hsa-mir-514a-1（OR=0.603，95%CI=0.413-0.879），hsa-mir-550a-1（OR=1.680，95%CI=1.034-2.703） and tumor T stage（OR=1.640，95%CI=1.126-2.389） were independent influence factors of early recurrence，with statistically significant differences（P<0.05）. Conclusion：Expression levels of hsa-mir-155，hsa-mir-331，hsa-mir-514a-1 and hsa-mir-550a-1 in HCC tissues and tumor T stage are independent factors influencing early recurrence of postop－erative HCC.