PARP-1抑制剂PJ34对前列腺癌细胞生物学行为的影响

Effects of poly （ADP-ribose） polymerase-1 inhibitor PJ34 on biological behaviors of prostate cancer cell

摘要:

目的：观察聚腺苷二磷酸核糖聚合酶-1[poly（ADP-ribose） polymerase-1，PARP-1] 抑制剂PJ34对前列腺癌PC-3细胞增殖、凋亡、迁移、侵袭等生物学行为的影响。方法：使用不同浓度PJ34处理PC-3细胞后，CCK-8法检测细胞增殖，Western blot检测PARP-1蛋白表达，annexin V-FITC/IP双染法检测细胞凋亡，Transwell法检测细胞迁移、侵袭等相关生物学行为的变化。结果：PJ34组分别作用72 h、48 h、24 h后，PARP-1蛋白水平均明显低于control组（t=4.647，P=0.009 7；t=4.816，P=0.008 5；t=17.28，P<0.000 1）。PJ34组凋亡率为（17.64±4.49）%，与control组凋亡率的（3.83±0.74）%相比，差异有统计学意义（t=5.261，P=0.006）。Control组和PJ34组细胞迁移差异有统计学意义（t=23.67，P<0.000 1），细胞侵袭差异有统计学意义（t=29.31，P<0.000 1）。结论：PJ34通过抑制PARP-1的表达抑制前列腺癌细胞PC-3的增殖、迁移和侵袭能力。

Abstract:

Objective：To investigate the effect of PJ34，poly（ADP-ribose） polymerase-1（PARP-1） inhibitor，on the biological behavior of human prostate cancer cell line PC-3，including cells proliferation，apoptosis，migration and invasion. Methods：PJ34 with different concentration was used to treat PC-3 cells，CCK-8 assay was used to detect cell proloferation，Western blot was used to detect the expression of PARP-1，annexin V-FITC/IP double-staining method was used to detect cell apoptosis，Transwell method was used to detect changes of cell biological behavior including migration and invasion. Results：After treatment for 72 h，48 h and 24 h，PRAP-1 levels in the PJ34 group were lower than those in the control group（t=4.647，P=0.009 7；t=4.816，P=0.008 5；t=17.28，P<0.000 1）. Apoptosis rate in the PJ34 group was （17.64±4.49）%，while in the control group was （3.83±0.74）%，with statistically signifi－cant differences（t=5.261，P=0.006）. Differences in migration had statistical significance in two groups（t=23.67，P<0.0001）. In addi－tion，cell invasion had statistically significant differences（t=29.31，P<0.000 1）. Conclusion：PJ34 can inhibit PC-3 cells' prolifera－tion，apoptosis and invasion by inhibiting expression of PRAP-1.