MMP-19在胃腺癌中的表达及与神经型钙黏素的相关性

Expression of MMP-19 and its correlation with N-cadherin in gastric adenocarcinoma

摘要:

目的：检测胃腺癌组织中基质金属蛋白酶-19（matrix metalloproteinase-19，MMP-19）的表达，探讨其与神经型钙黏素（N-cadherin，N-cad）的关联性，分析其预后价值。方法：收集在华北理工大学附属医院确诊为胃腺癌并行根治性手术治疗的患者64例作为研究对象，留取肿瘤组织作为观察组，留取正常胃黏膜组织作为对照组。采用免疫组化法检测2组中MMP-19、胃腺癌中N-cad的表达，采用Western blot检测2组中MMP-19蛋白的半定量表达情况。选择胃癌MGC803细胞系，建立siMMP-19组、空载体转染组和空白对照组，应用Western blot检测N-cad的表达。结果：免疫组化结果显示，MMP-19在胃腺癌组织中表达的阳性率明显高于正常胃黏膜组织（57.8% vs. 23.4%， χ2=15.68，P=0.001）。MMP-19在不同浸润深度（57.8% vs. 37.9%， χ2=8.59，P=0.001）、有无癌栓（83.3% vs. 47.80%， χ2=6.69，P=0.010）、淋巴结转移分组（27.7% % vs. 47.6%， χ2=5.21，P=0.023）中的表达差别有统计学意义。生存分析显示MMP-19的表达与生存时间有关（P=0.020）。Pearson 相关分析显示MMP-19与N-cad正相关（r=0.62，P=0.013）。Western blot结果显示观察组中MMP-19的半定量表达明显高于对照组（1.68±0.23 vs. 1.10±0.24， χ2=5.32，P=0.020）。siMMP-19组中N-cad的表达明显低于空载体转染组及空白对照组。结论：MMP-19在胃腺癌组织中表达升高，参与肿瘤的形成和进展。胃腺癌组织中MMP-19与N-cad正相关。检测MMP-19的表达可能对判断胃腺癌的预后有一定价值。

Abstract:

Objective：To investigate the expression of matrix metalloproteinase-19（MMP-19） in gastric adenocarcinoma，explore its correlation with N-cadherin（N-cad），and analyze its prognostic significance. Methods：In our study，64 patients with gastric adenocar－cinoma of operation were collected as the study objects in the Affiliated Hospital of North China University of Science and Technology. Tumor tissue was taken as the observation group and normal gastric mucosa tissue was taken as the control group. The expression of MMP-19 was detected by immunohistochemistry method in two groups. The expression of N-cad was detected by immunohisto－chemistry method in observation group. Semi quantitative expression of MMP-19 was detected by Western blot in two groups. Gastric cancer cell line MGC803 was selected. We established siMMP-19 group，empty vector transfection group and blank control group. The expression of N-cad was detected by Western blot. Results：The positive rate of MMP-19 was higher in gastric adenocarcinoma than that in normal gastric mucosa（57.8% vs. 23.4%， χ2=15.68，P=0.001） by immunohistochemistry method. The expression of MMP-19 had statistical significance in infiltration depth（57.8% vs. 37.9%， χ2=8.59，P=0.001），tumor thrombus（83.3% vs. 47.80%， χ2=6.69，P=0.010） and lymph node metastasis（27.7% % vs. 47.6%， χ2=5.21，P=0.023）. Expression of MMP-19 was related to survival time by survival analysis（P=0.020）. Positive correlation was found between MMP-19 and N-cad（r=0.62，P=0.013） by Pearson correlation analysis. Semi quantitative expression of MMP-19 was significantly higher in the observation group than that in the control group by Western blot（1.68±0.23 vs. 1.10±0.24， χ2=5.32，P=0.020）. Expression of MMP-19 was lower in siMMP-19 group than that in empty vector transfection group and blank control group. Conclusion：MMP-19 is highly expressed in gastric adeno－carcinoma，and it’s involved in tumor formation and development. In gastric adenocarcinoma，MMP-19 is positively correlated with N-cad. Detection of the expression of MMP-19 in gastric adenocarcinoma may be of certain value in predicting the prognosis.