原花青素对放射性心脏损伤大鼠血清cTn-I、SOD、MDA、GSH-px的影响

Effects of proanthocyanidins on serum cTn-Ⅰ, SOD, MDA, GSH-px in rats with radiation-induced heart injury

摘要:

目的： 观察原花青素（proanthocyanidins）对放射性心脏损伤（radiation-induced heart damage，RIHD）大鼠血清肌钙蛋白Ⅰ（cardiac troponinⅠ，cTn-Ⅰ）、超氧化物歧化酶（superoxide dismutase，SOD）、丙二醛（malondialdehyde，MDA）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-px）表达水平的影响，以及对RIHD大鼠的可能作用及作用机制。方法： 60只雄性远交群（sprague dawley，SD）大鼠，随机数字表法分为6组，每组10只，分别为正常对照组、单纯照射组、OPC小剂量（50 mg/kg）组、OPC中剂量（100 mg/kg）组、OPC大剂量（150 mg/kg）组、蓝莓（200 mg/kg）组。除正常对照组外，其余各组大鼠给予6 MV X线单次大剂量照射心脏，总剂量（total dose，Dt）20 Gy，OPC组和蓝莓组大鼠给予相应药物灌胃。放疗结束后4周，HE染色、Masson染色观察大鼠心肌组织病理改变，ELISA检测血清cTn-Ⅰ、SOD、MDA、GSH-px表达水平。结果： 与正常对照组比较，单纯照射组、OPC组、蓝莓组病理评分和心肌胶原容积分数（collagen volume fraction，CVF）评分升高（P<0.05）；与单纯照射组比较，OPC组、蓝莓组病理评分和CVF评分升高（P<0.05），差异均具有统计学意义。与正常对照组相比，单纯照射组、OPC组、蓝莓组cTn-Ⅰ、MDA表达水平升高，单纯照射组、OPC组、蓝莓组SOD、GSH-px表达水平下降；与单纯照射组相比，OPC组、蓝莓组cTn-Ⅰ、MDA表达水平升高，OPC组、蓝莓组SOD、GSH-px表达水平下降，差异均具有统计学意义（P<0.05）。结论： OPC可通过抑制氧化应激，下调RIHD大鼠血清cTn-Ⅰ、MDA表达水平，上调血清SOD、GSH-px表达水平，抑制RIHD大鼠心肌损伤，疗效优于蓝莓。

Abstract:

Objective: To observe the influence of proanthocyanidins (OPC) on serum cardiac troponinⅠ (cTn-Ⅰ), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-px) on the expression level of RIHD rats, as wells as their possible effects and mechanism of action. Methods: Sixty male sprague dawley (SD) rats were randomized into 6 groups, with 10 rats in each group, namely normal control group, simple irradiation group, OPC low dose (50 mg/kg) group, OPC middle dose (100 mg/kg) group, OPC high-dose (150 mg/kg) group, and blueberry (200 mg/kg) group. In addition to the normal control group, the rats in the other groups were given 6 MV X-ray single high-dose irradiation to the heart, with a total dose (Dt) of 20 Gy, and the rats in the OPC group and blueberry group were intragastrically administered the corresponding drugs. Four weeks after the radiotherapy, hematoxylin eosin (HE) staining and Masson staining were used to observe the pathological changes of myocardial tissue in rats, and enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of serum cTn-Ⅰ, SOD, MDA, GSH-px. Results: Compared with the normal control group, the pathological scores and myocardial collagen volume fraction (CVF) scores of the simple irradiationgroup, the OPC group, and the blueberry group increased (P<0.05); compared with the simple irradiation group, the pathological scores and CVF scores of the OPC group and the blueberry group increased (P<0.05), with significant differences. Compared with the normal control group, the expression levels of cTn-Ⅰand MDA in the simple irradiation group, OPC group, and blueberry group increased, while the expression levels of SOD and GSH-px decreased; compared with the simple irradiation group, the expression levels of cTn-Ⅰand MDA in the OPC group and blueberry group increased, while the expression levels of SOD and GSH-px decreased, with significant differences (P<0.05). Conclusion: OPC can inhibit the oxidative stress, down-regulate the expression levels of serum cTn-Ⅰand MDA in RIHD rats, and up-regulate the expression levels of serum SOD and GSH-px, thus inhibiting the myocardial damage in RIHD rats, with a better curative effect than the blueberry.