外周血中肺腺癌转移相关转录本1对口腔鳞状细胞癌的临床诊断价值

Clinical diagnostic value of metastasis-associated lung adenocarcinoma transcript 1 in peripheral blood of patients with oral squamous cell carcinoma

摘要:

目的： 探讨外周血中肺腺癌转移相关转录本1（metastasis-associated lung adenocarcinoma transcript 1，MALAT1）对口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）的临床诊断价值。方法： 采用荧光定量RT-qPCR方法检测OSCC患者术前外周血样本及配对术后癌组织中MALAT1的表达。结果： MALAT1在OSCC患者外周血中的平均表达水平明显高于健康志愿者（1.845±0.142 vs.0.642±0.090，P<0.001）；MALAT1在外周血中的表达水平与配对癌组织成正相关，相关系数为0.783（P<0.001）；MALAT1在外周血中的表达水平与肿瘤TNM分期（P=0.001）及细胞分化程度相关（P=0.010）；MALAT1对OSCC诊断的ROC曲线下面积为0.892，当截断值为0.788时，其诊断灵敏度为85%，特异度为81%。结论： MALAT1对OSCC具有较好的临床诊断价值，有望成为OSCC疾病诊断、预后判断的血清分子标志物。

Abstract:

Objective: To investigate the clinical diagnostic value of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in the peripheral blood of patients with oral squamous cell carcinoma (OSCC). Methods: Fluoresence quantitative RT-qPCR was used to detect the expression of MALAT1 in the preoperative peripheral blood samples and paired postoperative tumor tissues of patients with OSCC. Results: The mean expression level of MALAT1 in peripheral blood of patients with OSCC was significantly higher than that of healthy volunteers (1.845±0.142 vs.0.642±0.090, P<0.001). The expression level of MALAT1 in peripheral blood was positively correlated with the paired tumor tissues with a correlation coefficient of 0.783 (P<0.001). The expression level of MALAT1 in peripheral blood was correlated with tumor TNM stage (P=0.001) and cell differentiation (P=0.010). The area under the ROC curve of MALAT1 for the diagnosis of OSCC was 0.892.When the cutoff value was 0.788, the diagnostic sensitivity and specificity of MALAT1 were 85%and 81%respectively. Conclusion: MALAT1 has potential clinical value of diagnosing OSCC, and is expected to be a serum molecular marker for the diagnosis and prognosis of OSCC.