姜黄素对大鼠慢性细菌性前列腺炎的干预作用及其机制研究

Effects of curcumin on chronic bacterial prostatitis in rats and its mechanism

摘要:

目的：评价姜黄素对大鼠慢性细菌性前列腺炎（chronic bacterial prostatitis，CBP）炎性状态的影响及探讨其可能相关机制。方法：随机将40只SD大鼠分为姜黄素200 mg组、姜黄素100 mg组、氧氟沙星组、模型组及假手术组。采用大肠杆菌混悬液前列腺内注射的方法建立大鼠CBP模型，1个月后各治疗组给予药物治疗，持续12 d，电镜观察各组前列腺组织病理改变，real-time PCR测其环氧合酶-2（cyclooxygenase-2，COX-2）与诱导性一氧化氮合成酶（inducible nitric oxide synthase，iNOS） mRNA的表达，ELISA法检测肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）及Western blot测定核转录因子-?资B（nuclear factor κB，NF-κB）蛋白的表达水平。结果：①COX-2、iNOS mRNA及TNF-α在模型组中表达与假手术组比较都有升高（P=0.000，P=0.000，P=0.000），而各治疗组相对模型组而言其表达都有不同程度的降低（P<0.01），其中姜黄素200 mg组相对姜黄素100 mg组与氧氟沙星组表达量降低更为明显（COX-2：P=0.015，P=0.000；iNOS：P=0.000，P=0.000）。②采取姜黄素治疗的2组能够抑制NF-κB的表达，随着剂量提高作用越明显，且与COX-2、iNOS、TNF-α的表达呈正相关（rs=0.959，P=0.000；rs=0.945，P=0.000；rs=0.910，P=0.000），氧氟沙星组对NF-κB无明显影响。结论：姜黄素对大鼠CBP炎性状态有抑制作用，随剂量增加效果增强，其作用可能与抑制NF-κB的表达有密切关系。

Abstract:

Objective：To evaluate the influence of curcumin on the inflammation status of chronic bacterial prostatitis（CBP） in rats and to explore its possible mechanism. Methods：Totally 40 SD rats were randomly divided into following groups：curcumin 200 mg group，curcumin 100 mg group，ofloxacin group，model group and sham-operated group. CBP rat model was established by injecting escherichia coli strains into the prostate. After one month，drug was given to each treatment group for 12 d and the pathological changes of collected prostate samples were observed under electron microscope. The levels of cyclooxygenase-2（COX-2） and inducible nitric oxide synthase（iNOS） mRNA were characterized by real-time PCR and the levels of tumor necrosis factor-α（TNF-α） was detected by ELISA and the levels of tumor necrosis factor-α（NF-κB） was detected by Western blot. Results：①The levels of COX-2，iNOS mRNA and TNF-α protein were remarkable higher in model group than in sham-operated group（P=0.000，P=0.000，P=0.000）. The levels of COX-2，iNOS mRNA and TNF-α protein were decreased in each treatment group than in model group（P<0.01） and the de－crease was more obvious in curcumin 200 mg group than in curcumin 100 mg group and ofloxacin group（COX-2：P=0.015，P=0.000；iNOS：P=0.000，P=0.000）. ②Curcumin therapy can inhibit the level of nuclear factor kappa b（NF-κB） and the effect enhanced with the increase of the dose. The effect was positively correlated with the level of COX-2，iNOS and TNF-α（rs=0.959，P=0.000；rs=0.945，P=0.000；rs=0.910，P=0.000）. Ofloxacin exerted no effect on NF-κB. Conclusion：Curcumin can suppress the inflammation of CBP in rats and the effect enhances with the increase of the dose. The effect may have a close relationship with the level of NF-κB.