老年人群klotho G-395A、F352V与C370S基因多态性及组合与其血清klotho蛋白水平的相关性研究

Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing

摘要:

目的：探讨重庆市汉族老年人群Klotho G-395A、F352V与C370S 3个位点的单核苷酸多态性（Single nucleotide poly－morphism，SNP）及其组合与血清klotho蛋白水平的相关性。方法：随机选择汉族老年人390例，采用等位基因特异性引物PCR技术检测Klotho G-395A、F352V与C370S SNP，酶联免疫吸附试验（Enzyme-linked immunosorbent assay，ELISA）检测其血清klotho蛋白水平。结果：Klotho G-395A、F352V与C370S SNP各等位基因型携带者其血清klotho蛋白水平均无显著性差异（P>0.05）。基因组合分析研究发现Klotho G-395A、F352V与C370S SNP各组合等位基因型携带者其血清klotho蛋白水平差异性较大，其中Klotho G-395G+F352F+C370S携带者较Klotho G-395G+F352F+C370C携带者其血清klotho蛋白水平明显增高（P=0.056）。结论：Klotho G-395A、F352V与C370S SNP各等位基因型携带者其血清klotho蛋白水平无显著性差异，而组合等位基因型研究发现Klotho G-395G+F352F+C370S携带者较Klotho G-395G+F352F+C370C组合等位基因型携带者血清klotho蛋白水平增高有显著性趋势。

Abstract:

Objective：To investigate the association between serum klotho protein level and single nucleotide polymorphisms （SNPs） and combined genotypes of Klotho G-395A，F352V and C370S in elderly people of Han nationality in Chongqing. Methods：Unrelat－ed 390 elderly individuals from Chinese Han population （in Chongqing were selected.Single nucleotide polymorphisms of Klotho gene in the G-395A，F352V and C370S were tested by allele-specific primer（ASP） PCR，and serum klotho protein level was determined by enzyme-linked immunosorbent assay（ELISA）. Results：No significant difference was found in serum klotho protein level in the aged carrying allelic genotypes of Klotho G-395A，F352V and C370S SNP（P>0.05）. According to combined genotype analysis，the klotho protein level in the elderly individuals carrying Klotho G-395G+F352F +C370S was higher than that in the elderly individuals with Klotho G-395G+F352F+C370C（P=0.056）. Conclusion：No association of allelic genotypes of Klotho G-395A，F352V and C370S SNP with serum klotho protein levels is found.But a trend is found that serum klotho protein level in those with Klotho G-395G+F352F+C370S is higher than that in the elderly individuals with Klotho G-395G+F352F+C370C.