性激素结合球蛋白基因Asp327Asn多态性与2型糖尿病的相关性研究

Association of Asp327Asn polymorphism in the sex hormone-binding globulin gene with type 2 diabetes mellitus in Chinese Han population

摘要:

目的：研究中国汉族人群性激素结合球蛋白（Sex hormone-binding globulin，SHBG）基因Asp327Asn多态性与2型糖尿病（Type 2 diabetes mellitus，T2DM）发病风险的关系。方法：应用限制性片段长度多态性（Polymerase chain reaction-restriction fragment length polymorphism，PCR-RFLP）方法，对599例重庆及周边地区汉族人群（T2DM患者248例、正常对照351例） Asp327Asn进行基因分型。用稳态模型（Homeostatic model assessment，HOMA）评估胰岛素抵抗（HOMA-IR）和胰岛β细胞功能（HOMA-B）。结果：Asn等位基因的分布频率T2DM组低于正常对照组（分别为14.5％、20.1％）；与Asp等位基因携带者相比，Asn等位基因携带者患T2DM的风险显著降低（OR=0.62，95％CI为0.42~0.91，P=0.016）。结论：SHBG基因Asp327Asn位点Asn等位基因可能与低T2DM患病风险相关。

Abstract:

Objective：To investigate the association of a common functional polymorphism （Asp327Asn） in the sex hormone-binding globulin（SHBG）gene with type 2 diabetes mellitus （T2DM） in Chinese Han population. Methods：Asp327Asn polymorphism in 248 cases（T2DM group） and 351 controls （NC group） were genotyped by polymerase chain reaction-restriction fragment length polymor－phism （PCR-RFLP） technique.Homeostatic model assessment （HOMA） was used to assess beta cell function（HOMA-B） and in－sulin resistance （HOMA-IR）. Results：The variant Asn allele frequency was significantly lower in T2DM group than（14.5％，20.1％，respectively） that in NC group.Unconditional logistic regression analysis showed that the variant Asn allele was associated with a re－duced risk of T2DM（OR=0.62，95％CI:0.42~0.91，P=0.016）． Conclusion：This study suggests that the Asn allele in the SHBG gene might be related to a reduced risk of T2DM in the Chinese Han population.