胎儿骨髓间充质干细胞EGF受体或IGF受体的表达与其向上皮细胞分化的关系

The relation between the expression of EGF-R or IGF-1-R and epithelial differentiation in fetal bone marrow mesenchymal stem cells

摘要:

目的：观察骨髓间充质干细胞（Bone marrow mesenchymal stem cells，BMSCs）表达表皮生长因子受体（Epidermal growth factor-receptors，EGF-R）和胰岛素样生长因子1 受体（Insulin-like growth factor-1-receptors，IGF-1-R）与其向上皮细胞分化分化的关系。方法：取第3代（P3）-BMSCs分别用或不用含EGF、IGF-1的介质诱导培养；另取P5-BMSCs用EGF-R抗体或IGF-1-R抗体孵育4 h后，加入含或不含EGF、IGF-1的培养基培养。免疫组织化学SP法检测各组细胞表达EGF-R、IGF-1-R和细胞角蛋白（Cytokeratin，CK）的时间。结果：P3-BMSCs分别于无诱导培养的8 ～11 d、10～14 d和16～18 d首次表达EGF-R，IGF-1-R和CK;经EGF或IGF-1诱导后，EGF-R、IGF-1-R的表达较无诱导组提前2～4 d。P5-BMSCs经抗EGF-R或IGF-1-R抗体阻滞后，细胞表达CK的时间较对照晚2 d。结论：EGF-R和IGF-1-R的表达与培养的BMSCs向上皮细胞分化密切相关;体外培养的P4-BMSCs～P5-BMSCs为加入外源性EGF、IGF-1诱导其向上皮细胞分化的最佳时间点。

Abstract:

Objective：To observe the relation between the expression of EGF-R or IGF-1-R and epithelial differentiation in bone marrow mesenchymal stem cells（BMSCs）. Methods：The cultured passage 3（P3） BMSCs were induced or not induced by EGF or IGF-1；The P5-BMSCs were cultured with antibody of EGF-R or IGF-1-R for 4 h，and then induced or not induced by EGF or IGF-1.The expression time of EGF-R，IGF-1-R and CK in these cells was detected with immunohistochemical Sp method. Results：The first expression time of EGF-R，IGF-1-R and CK in un-induced P3-BMSCs was respectively on the 8th～11th d，10th～14th d and 16th～18th d. The expression time of EGF-R or IGF-1-R，which was induced by EGF or IGF-1，was 2 d～4 d earlier than the time in un-in－duced cells. When P5-BMSCs were blocked by antibody of EGF-R or IGF-1-R，the expression time of CK was 2 days later than that of control. Conclusion：The expression of EGF-R and IGF-1-R in BMSCs was closely correlated with their epithelial differentiation in vitro；The passages 4～5 BMSCs were best time for the addition of exogenous EGF、IGF-1 to induce BMSCs differentiation into ep－ithelial cell in vitro.