蒽环类药物耐药乳腺癌细胞中microRNA差异表达分析

Analysis of differential microRNA expression in anthracycline-resistant breast cancer cell lines

摘要:

目的：检测乳腺癌蒽环类药物耐药细胞株中微小RNA（microRNA，miRNA）的表达差异，探讨miRNA与乳腺癌化疗耐药的关系。方法：miRNA芯片检测乳腺癌蒽环类药物（阿霉素）耐药细胞MCF-7/ADM和其亲本细胞MCF-7的miRNA表达谱，找出差异表达的miRNA，并利用实时荧光RT-PCR法对miRNA芯片结果进行验证。结果：耐药细胞株MCF-7/ADM与亲本细胞株MCF-7相比有14个差异表达的miRNA，其中上调表达的有6个，下调表达的有8个；实时荧光RT-PCR进一步验证证实has-miR-221、has-miR-222、has-miR-106a在MCF-7/ADM细胞中表达显著上调，而has-let-7a、has-miR-421、has-miR-23b在MCF-7/ADM细胞中表达显著下调，与芯片的结果一致。结论：miRNA在乳腺癌蒽环类药物耐药细胞和其亲本细胞中差异表达，可能参与乳腺癌耐药的发生

Abstract:

Objective: To analyze the difference in microRNA expression in breast cancer cell lines of anthracycline anticancer drug resistance and explore the association between microRNA and drug resistance of breast cancer. Methods：The differentially expressed miRNAs between anthracycline anticancer drug-resistant breast cancer cell line MCF-7/ADM and its parental cell line MCF-7 were identified by miRNA profiling of these two cell lines using miRNA microarray；the results obtained by microarray profiling were vali－dated using real-time RT-PCR analysis. Results：Microarray analysis of MCF-7 to MCF-7/ADM cells identified 14 differentially ex－pressed genes，including 6 up-regulated and 8 down-regulated genes in MCF-7/ADM cells；real-time RT-PCR identified 6 microR－NAs that were differentially expressed between MCF-7 and MCF-7/ADM cells，including 3 up-regulated microRNAs：has-miR-221，has-miR-222，has-miR-106a，and 3 down-regulated microRNAs：has-let- 7a，has-miR -421，has-miR-23b in MCF-7/ADM cells.Conclusion：microRNA are differentially expressed in anthracycline anticancer drug-resistant breast cancer cell lines and its parental cell line，which suggests that microRNA may play a role in the development of drug-resistance in breast cancer cells.