大鼠肝部分切除后肝再生组织中Survivin基因的表达变化

Expression of Survivin gene in hepatic regeneration tissue of rat after partial hepatectomy

摘要:

目的：探讨Survivin在大鼠肝部分切除（Partial hepatectomy，PH）后肝再生过程中的作用及其意义。方法：用70%肝部分切除方法，建立Wister大鼠肝再生模型，在肝再生不同时间点，采用免疫组化及Western blot方法，检测肝组织中Survivin和细胞周期相关蛋白CyclinD1的表达。结果：在肝切除术后12~72 h，Survivin蛋白表达量明显增高，且在48 h达到峰值，细胞周期相关蛋白CyclinD1在术后12、24、48、72 h均表达上调，与Survivin蛋白表达具有高度相关性。结论：Survivin蛋白的高表达与肝部分切除后肝再生过程密切相关。

Abstract:

Objective：To investigate the effects and its significance of survivin gene in rat liver regeneration after partial hepatectomy. Methods：Wister rat liver regeneration model of 70% partial hepatectomy was established. The expressions of Survivin and cell cycle-related protein cyclinD1 were detected by immunohistochemistry in liver regeneration rat tissues at different time points. Results：The level of Survivin protein expression was significantly increased during 12~72 h after partial hepatectomy and reached its peak at 48 h after partial hepatectomy. The level of cyclinD1 was upregulated at 12，24，48 h，and 72 h after partial hepatectomy and highly correlated with survivin protein level. Conclusion：The high expression of Survivin protein is significantly correlated with the liver regeneration after partial hepatectomy.