Egr-1与TNF-α在口腔扁平苔藓病损中表达的研究

The study on the expression of Egr-1 and TNF-α in oral lichen planus

摘要:

目的：研究早期生长反应基因-1（Early growth response gene-1，Egr-1）和肿瘤坏死因子-α（Ｔumor necrosis factor-α，TNF-α）在口腔扁平苔藓（Oral lichen planus，OLP）组织中的表达及其是否存在相关性。方法：采用免疫组织化学法和Western blot技术检测30例正常口腔黏膜（Normal oral mucosa，NOM）组织与30例OLP组织中Egr-1和TNF-α的表达情况。结果：（1）上皮层中Egr-1、TNF-α在NOM组和OLP组均有表达，两组比较Egr-1表达无统计学意义（P＞0.05），TNF-α表达为OLP组高于NOM组（P＜0.05）；在固有层中Egr-1、TNF-α的表达均为OLP组高于NOM组（P＜0.05）。（2）Egr-1与TNF-α的表达量均为OLP组高于NOM组（P＜0.05）。（3）在OLP组中Egr-1、TNF-α表达呈正相关（r=0.447，P＜0.05）。结论：Egr-1和TNF-α是OLP发病的重要因素之一，Egr-1可能调控TNF-α的过量释放引起OLP的发病；两者的作用机制有待于进一步研究。

Abstract:

Objective：To investigate the expression of Egr-1 and TNF-α in normal oral mucosa（NOM） and oral lichen planus（OLP） and their correlation. Methods：Using immunohistochemistry and Western blot technique，the expressions of early growth response gene-1（Egr-1） and tumor necrosis factor-α（TNF-α） in the tissues samples（30 cases of NOM tissues and 30 cases of OLP tissues） were detected. Results：（1）In epithelial lamina Egr-1 and TNF-α had expressed both in NOM and OLP，the expression of Egr-1 was no significant difference in the two groups（P>0.05），the expression of TNF-α in OLP group was significantly higher than that in NOM group（P<0.05）；in lamina propria the expression of Egr-1 and TNF-α in OLP group was significantly higher than that in NOM group （P<0.05）. （2）The expressed quantity of Egr-1 and TNF-α in OLP group was significantly higher than that in NOM group（P<0.05）.（3）The expression of Egr-1 and TNF-α in OLP showed a positive correlation（r=0.447，P＜0.05）. Conclusion：Egr-1 and TNF-α were one of the most important factor of OLP，TNF-α excess release of OLP onset may be caused by Egr-1 regulation；The mechanism of action remains to be further studied.