登革病毒中国株E基因重组的生物信息学分析

Bioinformatics analysis of envelope gene recombination of Chinese dengue virus isolates

摘要:

目的：了解我国登革病毒（Dengue virus，DV）包膜蛋白基因（Envelope，E）重组的发生情况。方法：从GenBank收集共70个DV中国株的E基因序列，Clustal-W比对后，重组检测软件包RDP3检测E基因中可能存在的重组事件，并经Mega4构建系统发生树验证所检测的重组事件的可靠性和真实性。结果：DV-1型中E基因区存在3个重组事件，共4个重组子，分别是1995、1997、1999年和2004年分离的DV病毒株，在其它3个血清型的DV病毒株中没有检测到重组，也未检测到血清型间重组。结论：我国ＤＶ 1型血清型E基因区域存在基因重组。

Abstract:

Objective：To explore the recombination of envelope protein （E） gene of Chinese dengue virus （DV） isolates. Methods：70 Chinese DV E gene sequences available from GenBank were collected，and aligned with Clustal-W，then were incorporated into recombination detection program （RDP3） for potential recombination event detection. Phylogenetic trees constructed by Mega4 were further generated to confirm the recombination events. Results：Three intra-serotype recombination events，four recombinants isolated in 1995，1997，1999，and 2004，respectively，were confirmed in DV-1 E gene data. However，no recombination event was detected in other three serotypes in the data，so was the inter-serotype recombination. Conclusion：Recombination of E gene regions of Chinese dengue virus type 1 exists，although limited data were available.It highlights the necessity of employing more gene regions and se－quences in the analysis to fully detect the recombination in China.