双葛解酲丸对酒精性脂肪肝大鼠保护作用的实验研究

Experimental study on hepatic-protective effects of Shuangge Jiecheng Pill in rats of alcoholic fatty liver

摘要:

目的：观察双葛解酲丸对酒精性脂肪肝（Alcoholic fatty liver，AFL）模型大鼠肝组织乙醇脱氢酶（Alcohol dehydroge－nase，ADH）活力、血清酶活力、肝组织蛋白（Ｈepatic tissue protein，HTP）含量、脏器指数等的影响，探讨本处方对AFL的保护作用。方法：以56度红星二锅头酒灌胃（10 ml/kg，2次/d，连续8周）的方法建立AFL大鼠模型。60只大鼠随机平均分设空白对照组、模型对照组、硫普罗宁对照组（硫普罗宁片40 mg/kg）、双葛解酲丸（双葛）高剂量组、中剂量组和低剂量组[20、10、5 g生药/（kg·d）]，连续4周。结果：模型组血清r－谷氨酰转移酶（r-glutamyltransferase，GGT）、丙氨酸转氨酶（Alanine aminotransferase，ALT）、天冬氨酸转氨酶（Aspartate aminotransferase，AST）活力、尿酸（Ｕric acid，UA）、碱性磷酸酶（Alkaline phosphatase，AKP）及肝脏指数较空白对照组明显升高（P＜0.05），ADH活力、HTP、胸腺指数、体质量明显降低（P＜0.01），肝组织可见病理异常；给药后双葛组和硫普罗宁组上述指标改善有显著统计学意义、脂肪变性程度和炎症活动度记分显著下降（P＜0.01），以双葛高剂量组改善更明显（P＜0.01）。结论：双葛解酲丸能加速肝内乙醇代谢，减轻乙醇对肝细胞及大分子蛋白的损害，减轻脂肪在肝脏的蓄积从而保护肝脏功能，对AFL确有疗效；双葛解酲丸高剂量对上述指标的改善更优。

Abstract:

Objective：To observe the effects of Shuangge Jiecheng Pill on vigor of ADH，GGT，ALT，AST and hepatic tissue protein（HTP），UA，AKP，organ indexes，fatty degeneration degree and inflammation activity in rats of alcoholic fatty liver，and approach its hepatic protective effects. Methods：The model of alcoholic fatty liver disease rat was induced by intragastric administration. 60 rats were randomly divided into 6 groups: 10 were control group，10 model group，10 tiopronin （TP） group and 30 Shuangge Jiecheng Pill（SG） group with high dose（HD），middle dose（MD） and low dose（LD） respectively. SG group were treated with different dosages of Shuangge Jiecheng Pill（20，10，5 g crude drug/（kg·d） and compared with TP group administrated by tiopronin tablet（40 mg/kg）for 4 weeks. The changes of ADH，GGT，ALT，AST，HTP，UA，AKP，organ indexes，fatty degeneration degree and inflammation activity of the rats in the different experimental groups were observed. Results:Compared with the control group，GGT，ALT，AST，UA，AKP and the liver indexes in the model group were obviously increased（P＜0.05） while ADH，HTP，thoracic gland indexes and the body weights were decreased（P＜0.01） and the pathologic hepatic tissue was seen. After the experiment and compared with the model group，the liver indexes in the TP and SG group of HD，MD and LD were obviously changed（P＜0.01） and fatty degeneration degree and inflam－mation activity were lightened. And the indexes in SG group of HD were changed more obviously（P＜0.01） than those of SG group of MD and LD. Conclusion:Shuangge Jiecheng Pill is an effective method for alcoholic fatty liver disease by promoting the metabolism of alcohol，lessening the accumulation of fat in the liver，relieving the hepatocellular damage and resisting impairments to hepatic pro－tein resulting from alcohol. The effect of SG group of HD is significantly superior to the others.