结直肠癌中MMP-7、VEGF-D和VEGFR-3的表达相关性研究

Expressions of MMP-7，VEGF-D and VEGFR-3 in colorectal carcinoma and its clinic significance

摘要:

目的：通过检测结直肠癌中基质金属蛋白酶-7（Matrix metalloproteinase-7，MMP-7）、血管内皮生长因子-D（Vascular endothelial growth factor-D，VEGF-D）和血管内皮生长因子受体-3（Vascular endothelial growth factor receptor-3，VEGFR-3）的表达，探讨MMP-7与淋巴管生成因子的关系。方法：应用免疫组化法检测124例结直肠癌和70例正常黏膜组织中MMP-7、VEGF-D和VEGFR-3的表达，比较两种组织中3种蛋白表达的差异，分析其与结直肠癌临床病理指标的关系及三者的相关性。结果：MMP-7、VEGF-D和VEGFR-3表达的阳性率在结直肠癌中明显高于黏膜组织（P<0.001），MMP-7的表达与结直肠癌的肿瘤体积、Dukes分期、浸润深度及淋巴结转移有关（P<0.05）；VEGF-D和VEGFR-3的表达与肿瘤体积、淋巴结转移有关（P<0.05）。结直肠癌中MMP-7和VEGF-D、VEGFR-3的表达呈正相关性（均P=0.01）。MMP-7和VEGF-D、VEGFR-3的高表达与患者的不良预后相关（P<0.05）。结论：结直肠癌中MMP-7的表达与VEGF-D、VEGFR-3的表达密切相关，可能在淋巴管新生中起协同作用，促进淋巴道转移。联合检测MMP-7、VEGF-D和VEGFR-3可能对判断结直肠癌的预后有一定的指导意义。

Abstract:

Objective：To study the expressions of matrix metalloproteinase-7（MMP-7），vascular endothelial growth factor-D （VEGF-D） and （Vascular endothelial growth factor receptor-3）（VEGFR-3） in colorectal carcinomas and relationship between MMP-7 ex－pression and lymphangiogenesis. Methods：124 primary tumors with colorectal carcinoma were studied，together with 70 normal col－orectal tissues by immunohistochemistry. Results：The expressions of MMP-7，VEGF-D and VEGFR-3 were higher in the primary tu－mors than those in mucosa. MMP-7 expression was associated with tumor volume，Dukes stage，invasive depth and lymph node metastasis. VEGF-D and VEGFR-3 expressions were associated with tumor volume and lymph node metastasis. There was positive correlation between MMP-7，VEGF-D and VEGFR-3 in primary tumors. The higher expressions of MMP-7，VEGF-D and VEGFR-3 indicated worse prognosis in colorectal carcinoma. Conclusion：MMP-7 overexpression was closely related to the expressions of VEGF-D and VEGFR-3 that might be involved in the lymphangiogenesis. MMP-7，VEGF-D and VEGFR-3 might be helpful to predict the prognosis of colorectal carcinoma.