体质指数与大鼠急性肺损伤模型的肺损伤程度相关性研究

Correlation of body mass index and lung injury in rat models of acute lung injury

摘要:

目的：急性肺损伤（Acute lung injury，ALI）发病机制复杂，死亡率高。本研究从大鼠体质指数（Body mass index，BMI）及血脂水平角度出发探讨脂质代谢是否在ALI发病机制中起着调控作用及其可能途径。方法：36只雄性SD（Sprague-dawley，SD）大鼠随机平均分为对照组（C组）、模型组（OA组）、高脂肥胖组（C-H组）、高脂肥胖模型组（O-H组），造模后预定时间点处死大鼠并取肺组织作湿重/干重比值（W/D）、肺组织病理学观察、肺组织核因子-κB（Nuclear factor -kappa B，NF-κB）表达水平，及测定炎症因子白细胞介素-1β（Interleukin-1β，IL-1β）、肿瘤坏死因子α（Tumor necrosis factor α，TNF-α）等。结果：O-H组在肺损伤病理学评分以及IL-1β、TNF-α 3项指标均较OA组程度升高（P＜0.05），前者肺组织NF-κBmRNA表达水平较后者明显上调，C-H组较C组相比也存在上述指标的显著差异。结论：在相同单位剂量油酸的诱导下，从mRNA及炎症因子水平提示了BMI水平与肺损伤程度呈正相关，是影响肺损伤程度的独立因子，为临床防治提供一定的参考价值。

Abstract:

Objective:To investigate the correlation between BMI and lung injury degree in acute lung injury of rats. Methods：A total of 60 male sprague-dawley （SD ）rats were randomly divided into control group（C），Oleic acid-treated group（OA），control-hperlipi－demia（C-H） group，and Oleic acid-hperlipidemia（O-A） group. Acute lung injury was induced by injection of OA，O-A（does，0.15 ml/kg） via intravenous vein. Rats were killed at scheduled time-points and the Lung tissues were removed for lung pathology and determination of wet/dry weight ratio（W/D），nuclear factor-kb（NF-κB） expression，inflammatory cytokines interleukin-1β（IL-1β），and tumor necrosis factor α（TNF-α）. Results：In O-H group，the pathology of lung injury score，IL-1β and TNF-α were significant－ly increased，as compared with the OA group （P＜0.05）. The above lung tissue expression of NF-κB mRNA in O-H group were also significantly increased than OA group. Conclusion:Under the same dose of oleic acid induction，the mRNA and the level of inflam－matory cytokines suggest that BMI levels and lung injury were positively correlated with each other.