炎症标志物IL-3在PCOS患者外周血的表达水平及临床意义

Peripheral circulating expression of IL-13 in women with Polycystic Ovary Syndrome

摘要:

目的：观察白细胞介素(interleukin, IL)13在多囊卵巢综合征(Polycystic ovary syndrome, PCOS)患者外周血中的表达水平,并分析其与患者体重指数(BMI)、生殖相关激素、血糖及血脂的相关性。方法：临床对照试验。研究对象分为三组：PCOS肥胖组(40例)、PCOS非肥胖组(35例)和正常对照组(39例)。测量各组研究对象的体重、身高、腰围及臀围,并计算BMI及腰臀比(WHR)。分别用酶联免疫吸附试验(ELISA)测定血清IL-13水平；应用化学发光法检测空腹血糖(FBG)、空腹胰岛素(Ins)、胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平；放射免疫测定：卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕酮(P)、催乳素(PRL)、睾酮(T)。结果：PCOS肥胖组外周血IL13的表达高于非肥胖组(P = 0.000),且二者均高于正常对照组 (P = 0.000；P = 0.019)。Ins、LDL、HDL、TG、TC、IR、E2、FSH、P等指标的血清水平无统计学差异；而WHR、BMI、FBG、LH、T等指标血清值PCOS肥胖组、非肥胖组均高于对照组。相关回归分析显示IL13与BMI (R=0.527, P=0.000)中度相关；与FBG (R=0.449, P=0.000)及T (R=0.452, P=0.000)有低度相关性；而与WHR (R=0.272, P=0.021)及 LH (R=0.247, P=0.037)无明显相关性。结论：炎症标志物IL13在PCOS患者外周血中表达明显升高,并与BMI、FBG及T等指标存在正相关。

Abstract:

Objective To observe the expression of interleukin13 (IL13) in Peripheral circulating of polycystic ovary syndrome (PCOS) patients, and analyze the relevance with the body mass index (BMI), reproductive relevant hormone, fasting blood glucose (FBG) and blood fat. Methods Clinical control trial was carried out. All objects of study were assigned to three groups: PCOS with obesity (40 cases), PCOS with non-obesity (35 cases) and normal control (39 cases). The body weight, body height, waist circumference and hip circumference were measured and BMI and waist/hip ratio (WHR) were calculated. ELISA kit for IL-13 was used to test the expression in peripheral circulating. Chemiluminescence’s method was applied to detect FBG, fasting insulin (Ins), cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) level for the three group objects. Reproductive hormones including follicle-stimulating hormone (FSH), corpus luteum erythropoietin (LH), estradiol (E2), progesterone (P), prolactin (PRL) and testosterone (T) were assayed by Radiation immunoassays (RI). Results The blood level of IL13 in PCOS with obesity was higher than that in PCOS with non-obesity (P = 0.000), and there were significance for both of all compared with that in normal control group (P = 0.000；P = 0.019). Ins, LDL, HDL, TG, TC, IR, E2, FSH and P serum levels were no statistical difference; and WHR, BMI, FBG, LH and T index serum value, both of the PCOS with obesity group and PCOS non-obesity group are higher than those in the control group. There was a moderate correlation for IL13 with BMI(R=0.527, P=0.000) and low-grade correlation with FBG (R=0.449, P=0.000) and T (R=0.452, P=0.000). Conclusion Inflammatory marker IL13 in the peripheral blood PCOS patients increased significantly, and has a positive correlation with BMI, FBG and T indexes.