EGCG联合紫杉醇对肺癌A549细胞增殖、凋亡的影响

Effects of (-)-epigallocatechin gallate combined with on Paclitaxel theproliferation and apoptosis of human lung cancer cell line A549

摘要:

目的：研究表没食子儿茶素没食子酸酯［(-)-Epigallocatechin gallate,EGCG］及紫杉醇单独使用和联合使用时对肺癌A549细胞的形态、活性、细胞凋亡以及相关信号通路的影响。方法：不同浓度的EGCG、紫杉醇单用及联合应用在不同时间作用于肺癌A549细胞，用细胞集落形成实验检测细胞活力、WST-1法检测细胞增殖能力、Hoechst-33342荧光法检测细胞凋亡的情况、流式细胞仪检测细胞凋亡率、Western blot检测内质网应激标志蛋白GRP78表达。结果：①EGCG和紫杉醇均能抑制肺癌A549细胞的增殖；EGCG与紫杉醇联合的抑制率高于单用EGCG或紫杉醇组（P＜0.05）。②EGCG和紫杉醇均可诱导细胞凋亡，2种药物联用后，诱导细胞凋亡作用显著增强。③蛋白免疫印迹实验显示EGCG和紫杉醇联合可能是通过内质网应激信号通路发挥作用。结论：EGCG、紫杉醇均可抑制人肺癌A549细胞的增殖、诱导细胞的凋亡，两者联合应用具有协同作用，能显著增强细胞凋亡作用。

Abstract:

Objective:To study the effects of combined and exclusive application of (-)-epigallocatechin gallate (EGCG) and Paclitaxel on the morphology,activity,apoptosis and possible signal pathway of human lung cancer cell line A549.Methods:EGCG and Paclitaxel with different concentrations were applied to human lung cancer cell line A549 exclusively and in combination at different periods.The cell activity was examined by cell colony formation assay,cell proliferation by WST-1 assay,cell apoptosis by Heochst-33342 test,apoptosis rate by flow cytometry and expressions of GRP78 by western blot.Results:Both EGCG and Paclitaxel can inhibit the proliferation of lung cancer cell line A549.The inhibiting effect was more obvious if the two drugs were used in combination (P<0.05).Both EGCG and Paclitaxel can induce the apoptosis of lung cancer cell line A549.The inducing effect was more obvious if the two drugs were used in combination.The results of western blot indicated that the two drugs may exert the effects through endoplasmic reticulum stress signal pathways.Conclusion:Both EGCG and Paclitaxel can inhibit the proliferation and induce the apoptosis of A549 cells,more obvious effects can be achieved if the two drugs are used in combination.