当归多糖对电离辐射致小鼠骨髓单个核细胞凋亡及氧化损伤的影响

Effects of Angelica polysaccharide on the apoptosis and oxidative damage in bone marrow mononuclear cells of ionizing radiation injured mice

摘要:

目的：探讨当归多糖（Angelica polysaccharides，APS）对电离辐射引起小鼠骨髓单个核细胞（Bone marrow mononuclear cell，BMNC）凋亡及氧化损伤的影响，旨在阐明APS对辐射性造血损伤的保护作用。方法：直线加速器4.0 Gy一次性全身均匀X射线照射C57BL/6小鼠，建立小鼠放射损伤动物模型。照射后连续7 d腹腔注射不同剂量APS或生理盐水（Normal saline，NS），并于照射后第1、3、7天处死小鼠，提取BMNC，流式细胞术检测细胞周期，细胞凋亡率以及免疫细胞化学法检测P53的表达；比色法检测其超氧化物歧化酶（Superoxide dismutase，SOD）活性以及丙二醛(Maleic Dialdehyde，MDA)含量。结果：与正常组相比，NS组G0/G1期细胞比例及凋亡率升高；SOD活性明显降低以及MDA含量明显升高；P53有表达。2 mg/kg APS组和8 mg/kg APS组均能降低G0/G1期细胞比例以及细胞凋亡率，降低突变型P53的表达，提高SOD活性，降低MDA含量。结论：APS可抑制辐射引起的细胞凋亡，还具有抗细胞氧化损伤的作用。

Abstract:

Objective: To study the effects of Angelica polysaccharide(APS) on the cell apoptosis and oxidative damage in bone marrow mononuclear cells (BMNC) of ionizing radiation injured mice so as to illuminate the protective effects of APS against radioactive injured haematogenesis.Methods:Linear accelerator irradiated C57BL/6 mice with 4.0 Gy X ray one time were used to establish animal radiation injured models.Then the mice were given different dosages of APS or normal saline(NS) for 7 days continuously by intraperitoneal injection.These mice were killed and BMNC was extracted after 1,3,and 7 days of injection.The cell cycle and apoptosis rate were detected by flow cytometry and the expression of P53 was detected by immunocytochemistry.The activity of superoxide dismutase(SOD) and content of Maleic Dialdehyde(MDA) were detected by the method of colorimetric assay.Results:Compared with those in the normal group,the percentage of G0/G1 phase,apoptosis rate,the content of MDA and the expression of P53 were increased significantly,while the activity of SOD was obviously decreased in NS group; in both 2 mg/kg APS group and 8 mgkg APS group,the percentage of G0/G1 phase,apoptosis rate,the content of MDA and the expression of P53 were decreased,while the activity of SOD was increased.Conclusion: APS can inhibit cell apoptosis caused by radiation and plays an important role in resisting oxidative damage.