普通冰箱4 ℃短期保存小鼠骨髓单个核细胞的研究

Research on short term preservation of mouse bone mononuclear cell in the ordinary refrigerator at the temperature of 4 ℃ 

摘要:

目的：建立小鼠骨髓单个核细胞短期保存简便有效的方法。方法：采用密度梯度离心法获得小鼠骨髓单个核细胞，DMEM培养液为小鼠骨髓单个核细胞冷藏营养液，直接置骨髓单个核细胞于4 ℃冰箱中保存，分别在当天至60 h选择性观察细胞形态，测其细胞回收率、活力及细胞周期。结果：小鼠骨髓单个核细胞置4 ℃冰箱保存2 d，活细胞率达80%以上；细胞回收率达70%以上；细胞周期与对照组相比无明显差别。结论：4 ℃冰箱能在2 d内保存小鼠骨髓单个核细胞，是一种简便易行的短期保存方法。

Abstract:

Objective:To find a simple and effective way of preserving bone mononuclear cells at short-term.Methods:Bone mononuclear cells from mice were isolated by density gradient centrifugation.DMEM culture solution was employed as the nutrient solution and bone mononuclear cells were preserved in the refrigerator at the temperature of 4℃.The form,recovery rate,viability and cycle of bone mononuclear cells were observed and detected optionally from the time of being preserved to 60 h after the preservation.Results:The living cell rate was over 80% and the cell recovery rate was over 70% after being preserved in the refrigerator at the temperature of 4 ℃ for two days.Furthermore,the cell cycle was similar to that of control group (P>0.05).Conclusion:Refrigerator at the temperature of 4 ℃ can safely preserve bone mononuclear cells within two days,which offers a simple and effective way of preserving.