不同温度下含川芎嗪UW液保存对异体神经再生影响的实验研究

Experimental study of the effects of different temperatures on the regeneration of allogeneic nerves preserved in UW solution with Tetramethylpyrazine

摘要:

目的：探讨不同温度对含川芎嗪UW液保存的大鼠异体神经再生的影响。方法：取3月龄雄性清洁级成年健康Wistar大鼠60只，体重200～250 g，随机分为A、B、C、D、E组5组(n=12)；取3月龄雄性清洁级成年SD大鼠18只，体重200～250 g，切取双侧坐骨神经干约15 mm，于4 ℃、-20 ℃、-80 ℃下，用含川芎嗪的UW液保存4周后，作为A、B、C组神经修复供体。D组为新鲜异体神经移植组；E组为新鲜自体神经移植组。术后4、8、12周行大体观察，术后12周行电生理检测，光镜、电镜观察神经再生情况。结果：术后12周腓肠肌湿重、电生理检测，A、E两组优于其他组，A、E两组结果相近，但组间有统计学意义(P＜0.05)；术后12周，A、E组组织形态学观察结果相似。结论：同种异体神经在4 ℃条件下经含川芎嗪的神经保存液保存可改善神经再生效果。

Abstract:

Objective:To investigate the effects of different temperatures on the regeneration of allogeneic nerves of rats preserved in UW solution with Tetramethylpyrazine(TMP).Methods:Totally 60 Wistar rats (male,weighing 200～250 g) were divided into five groups randomly (groups A,B,C,D and E,n=12).The sciatic nerve trunks of 18 SD rats (male,weighing 200～250 g) were incised at about 15 mm and were preserved in UW solution with TMP at different temperatures (group A:4 ℃,group B:-20 ℃,group C:-80 ℃) for fours weeks to repair the sciatic nerve defect of SD rats.Group D was allogeneic nerve graft group and Group E was autologous nerve graft group.General observation was performed at 4,8,12 weeks postoperatively while electrophysiological check,light and electron microscope check were conducted at 12 weeks postoperatively.Results:The results of A、E groups in the electrophysiological test and gastrocnemius weight test were better than those of the other groups,however,statistical differences were observed between group A and E (P＜0.05).Group A and E displayed similar results in morphology at 12 weeks after the operation.Conclusion:The regeneration of allogeneic nerves preserved in UW solution with TMP at 4 ℃ can be improved.