肿瘤-睾丸基因在中国人群髓母细胞瘤中的表达

Cancer-testis gene expression in medulloblastoma of the Chinese population

摘要:

目的：研究肿瘤-睾丸（Cancer-testis,CT）基因在中国人群髓母细胞瘤中表达的情况。 方法：收集25例髓母细胞瘤病例，分别采用RT-PCR和免疫组化检测髓母细胞瘤CT基因和蛋白的表达，并结合分型进行分析。 结果：〖HTSS〗non X-CT抗原之间相比，肿瘤分型之间的差异两两之间具有统计学意义（P＜0.05）。25例病例至少表达1个CT基因。GAGE是最常表达的CT基因，25例病例有17 例（68%）存在。19/20（95%）的病例中发现存在X-CT基因的共表达，而只有1/18（5.6%）的病例存在non X-CT基因的共表达。所有病例中单克隆抗体CT 7-33（ MAGEC1）是染色阳性反应最多的蛋白。对CT基因mRNA的表达和患者的临床参数（如年龄及临床结果）进行了统计学分析，并显示两者之间无显著相关性。结论：在分子水平上，CT抗原在10个CT基因中至少有1个存在。很多CT基因有mRNA水平的表达，却很少有蛋白水平的表达。

Abstract:

Objective:To study the cancer testis (CT) gene expression in medulloblastoma of the Chinese population.Methods:Totally 25 medulloblastoma cases were collected. RT-PCR and immunohistochemical methods were used to detect medulloblastoma CT gene and protein expression respectively, combined with genotyping analysis. Results:Tumor types were statistically different between each other in non X-CT antigens(P<0.05). CT gene was expressed in at least 1 case among 25 cases. GAGE was the most commonly expressed CT gene,existing in 17 cases (68%). CT-X gene co expression was found in 19/20 cases (95%) while non X-CT gene co expression in 1/18 cases (5.6%). Among all cases, monoclonal antibody CT 7-33 (MAGEC1) was the most positive protein staining. The CT gene mRNA expression and clinical parameters (such as age and clinical outcome) were statistically analyzed, displaying no significant correlation.Conclusion：At the molecular level, CT antigen exists in at least one CT gene. Many CT genes have mRNA expression,but few have protein expression.