超声联合紫杉醇微泡对卵巢癌细胞株A2780/DDP增殖和凋亡的影响

Effects of ultrasound plus Paclitaxel loaded microbubbles on the proliferation and apoptosis of ovarian carcinoma A2780/DDP cells

摘要:

目的：探讨超声联合紫杉醇微泡对人卵巢癌细胞A2780/DDP细胞的增殖和凋亡的影响。方法：将人卵巢癌细胞株A2780/DDP随机分为5组：对照组、紫杉醇组、紫杉醇+超声组、紫杉醇微泡组和紫杉醇微泡+超声组。采用噻唑蓝（Methyl thiazolyl tetrazolium,MTT）法检测作用24、48 h和72 h后卵巢癌细胞的增殖活性，并用Hochst 33258和TUNEL染色法检测细胞凋亡情况。结果：MTT法检测显示紫杉醇微泡+超声组24、48 h和72 h抑制率分别为（37.20±2.01）%、（51.60±2.10）%和 （57.47±2.85）%，明显高于其他各处理组（P＜0.05）；Hochst 33258和TUNEL染色均显示紫杉醇微泡+超声组凋亡率最高，作用24 h后的凋亡率为(46.10±4.15)%，明显高于其余各组（P＜0.05）。结论：超声联合紫杉醇微泡能明显抑制人卵巢癌细胞A2780/DDP的增殖和诱导其凋亡。

Abstract:

Objective:To evaluate the proliferative and apoptosis effects of Paclitaxel loaded microbubbles plus ultrasound on ovarian cancer A2780/DDP cell strain. Methods:A2780/DDP cell strain was randomly divided into five groups:control group, Paclitaxel group,Paclitaxel plus ultrasound irradiation group,microbubbles loaded with Paclitaxel group and microbubbles loaded with Paclitaxel plus ultrasound irradiation group. The proliferative activity of human ovarian cacinoma cell line A2780/DDP was measured by MTT assay at 24, 48 h and 72 h after the treatment and the apoptosis was analyzed by Hochst 33258 and TUNEL staining methods.Results:Resutls from MTT showed that the inhibitory rate of Paclitaxel loaded microbubbles plus ultrasound irradition group were (37.20±2.01)%,(51.60±2.10)% and(57.47±2.85)% respectively at 24, 48 h and 72 h after the treatment, significantly higher than those in the other groups (P<0.05). Results from Hochst 33258 and TUNEL staining indicated that the apoptosis rate was (46.10±4.15)% in microbubbles loaded with Paclitaxel plus ultrasound irradiation group at 24 h after the treatment,obviously higher than those in the other groups (P<0.05). Conclusion:Paclitaxel loaded microbubbles plus ultrasound irradiation can suppress the growth and induce the apoptosis of A2780/DDP cell.