抑制NFBD1的表达对鼻咽癌化疗敏感性的影响

Effect of siRNA mediated NFBD1 depletion on chemosensitivity in nasopharyngeal carcinoma cells

摘要:

目的：研究抑制NFBD1表达后，对鼻咽癌（Nasopharyngeal carcinoma，NPC）细胞化疗敏感性的影响。方法：将NFBD1 siRNA导入NPC细胞系，采用RT-PCR和蛋白质印记法检测siRNA对NPC细胞NFBD1表达的抑制效果；NFBD1 siRNA转染后，MTT法分析NPC细胞对化疗药物敏感性的影响。结果：NFBD1 siRNA转染NPC细胞后，能高效、特异地在mRNA水平及蛋白质水平抑制NFBD1的表达；MTT结果分析表明，NFBD1 siRNA转染后，能显著提高NPC细胞系对化疗药物的敏感性。结论：NFBD1 siRNA转染后抑制NPC NFBD1表达，可显著提高其对化疗药物顺铂的敏感性。

Abstract:

Objective:To investigate the effect of siRNA-mediated nuclear factor with BRCT domains protein 1(NFBD1)depletion on chemosensitivity in nasopharyngeal carcinoma cells. Methods：Using siRNA to inhibit expression of NFBD1 in nasopharyngeal carcinoma cell lines HNE-1(poorly differentiated squamous-cell carcinoma) and CNE-1(goodly differentiated squamous-cell carcinoma), RT-PCR and Western bolt analysis were used to verify the inhibitory effect of siRNA against NFBD1. MTT assay was used to determine the effect of NFBD1 depletion on the chemosensitity of nasopharyngeal carcinoma cells. Results：siRNA could specifically and efficiently deplete NFBD1 in nasopharyngeal carcinoma cells. Survival rate in the NFBD1 siRNA group was significantly degraded,compared with that in blank group and the control siRNA group. Conclusion： SiRNA-mediated NFBD1 depletion can significantly enhance the chemosensitivity of nasopharyngeal carcinoma cells to chemotherapeutic agents such as cisdiamminedichloroplatinum(CDDP).