肝细胞生长因子在高氧暴露新生鼠肾组织中表达的变化

Expression changes of hepatocyte growth factor in the renal tissue of neonatal rats exposed to hyperoxia

摘要:

目的：观察高氧暴露下新生大鼠肾组织结构的变化和肝细胞生长因子（Hepatocyte growth factor，HGF）表达的变化。方法：采用高氧暴露致新生大鼠肺损伤模型，将出生12 h内的72只SD大鼠随机分成2组，Ⅰ组：正常空气；Ⅱ组：高氧（90%±5%氧气）。于3、7、14 d各处死8只取标本，用苏木精 伊红（Hematoxylin-eosin,HE）染色法观察肾组织结构变化；用免疫组织化学法检测HGF蛋白表达水平；用逆转录聚合酶链反应（Reverse transcription polymerase chain reaction,RT-PCR）检测HGF mRNA表达强度。结果：①与Ⅰ组比较，Ⅱ组可见系膜细胞增生，肾小管上皮细胞肿胀，炎性细胞浸润。随时间延长，病理变化愈明显。②与Ⅰ组比较，Ⅱ组3 d时HGF蛋白表达水平增强（P<0.05），7 d时明显增强（P<0.05），14 d时也增强（P<0.05），但与7 d相比，增强趋势减弱；③HGF mRNA表达强度与其蛋白表达水平变化趋势相似。结论：HGF在高氧暴露致新生大鼠肾组织损伤中表达增强。

Abstract:

Objective:To examine the changes of the renal tissue structure and the expression changes of hepatocyte growth factor(HGF) in the renal tissue of neonatal rats exposed to hyperoxia. Methods: Neonatal rat lung injury model was induced by exposing to hyperoxia.Seventy-two neonatal sprague-dawley(SD)rats were randomly divided into groupⅠ(n=36) and groupⅡ(n=36) within 12 h after birth. The rats in group Ⅰwere exposed to normal air while those in groupⅡ to hyperoxia. Eight rats in each group were killed on 3th,7th,14th d after birth to make the samples. The changes of the renal tissue structure were examined by hematoxylin-eosin (HE) staining method;the protein expressions of HGF in the renal tissue were detected by immunohistochemical method;the mRNA expression intensities of HGF were measured by reverse transcription polymerase chain reaction (RT-PCR). Results: ①Mesangial cell proliferation,renal tubular epithelial cells swelling and inflammatory cells infiltration were observed in group Ⅱcompared with those in groupⅠ.The pathological changes became more and more obvious with the passage of time.②The protein expression levels of HGF were enhanced on 3th d in groupⅡ compared with those in groupⅠ (P<0.05),with more enhanced effect on 7th and on 14th d(P<0.05). However,the enhanced tendency on 14th d was weaker than that on 7th d. ③HGF mRNA expression intensities were similar to the changing tendency of their protein expression levels. Conclusion:The expression levels of HGF are enhanced in hyperoxia induced renal tissue injury.