西妥昔单抗联合紫杉醇对人乳腺癌MCF-7细胞生长抑制作用的实验研究

Study on the growth inhibition effect of Cetuximab combined with Paclitaxel on human breast cancer cell line MCF-7

摘要:

目的：观察表皮生长因子受体(Epidermal growth factor receptor,EGFR)单克隆抗体西妥昔单抗（Cetuximab）联合紫杉醇（Paclitaxel,PTX）对人乳腺癌MCF-7细胞的生长抑制作用。方法：实验分为对照组、Cetuximab组、PTX组、联合用药组。用MTT法检测Cetuximab、PTX及Cetuximab与PTX不同联合给药方案对MCF-7细胞的生长抑制率，流式细胞术检测各组细胞的凋亡率和周期分布，免疫荧光法及RT-PCR法检测PTX组和对照组细胞的EGFR蛋白及mRNA的表达。结果：MTT法检测发现PTX对MCF-7细胞的抑制作用呈剂量依赖性；Cetuximab与PTX不同的联合给药方案对MCF-7细胞的抑制强度不同，先予PTX再予Cetuximab可见明显的协同作用。与对照组相比，其余3组的细胞凋亡率均有所增加，但联合用药组较单药组更明显，差异有统计学意义（P<0.05）。与对照组比较，PTX组细胞的EGFR蛋白、mRNA的表达均减少（P<0.05）。结论：人乳腺癌MCF-7细胞对PTX敏感；PTX对MCF-7细胞的抑制作用呈剂量依赖性；Cetuximab和PTX联用可明显抑制乳腺癌MCF-7细胞的生长，促进其凋亡，二者联用具有协同作用。

Abstract:

Objective: To observe the growth inhibition effect of the single clone antibody of epidermal growth factor receptor (EGFR) Cetuximab combined with Paclitaxel (PTX) on human breast cancer cell line MCF-7.Methods: The experiment involved four groups: control group, Cetuximab group,PTX group and combination group. The inhibitory rates of Cetuximab,PTX and different combinations of Cetuximab and PTX on MCF-7 cells were examined by MTT assay and the apoptosis rate and cell cycle distribution were analyzed by flow cytometry. The immunofluorescence and RT-PCR were used to evaluate the EGFR protein and mRNA expression in control group and PTX group. Results:PTX can exert inhibitory effect on MCF-7 cells in a dose dependent manner based on the results from MTT assay. MTT assay showed that different combination schemes exerted different inhibitory effects; the synergistic effect was seen in the scheme of MCF-7 cells first treated with PTX and then Cetuximab. The apoptosis rate of the other three groups was increased compared with that of control group and the effect was more evident in combination group than in single agent groups (P<0.05).The EGFR protein and mRNA expressions reduced in PTX group compared with those in control group (P<0.05).Conclusion：Human breast cancer cell line MCF-7 was sensitive to PTX and PTX can exert inhibitory effect on MCF-7 cells in a dose dependent manner. The combination of Cetuximab and PTX can increase the apoptosis and decrease the proliferation of breast cancer cell line MCF-7 with a synergistic action.