早期母子隔离应激对幼鼠不同癫痫模型易感性的影响

Influence of early maternal isolation stress on the susceptibility of different epilepsy models in infantile rats

摘要:

目的：探讨早期母子隔离应激对幼鼠氯化锂-匹罗卡品和杏仁核电刺激点燃诱导癫痫模型易感性的影响。方法: 66新生SD大鼠幼鼠用于实验，随机分成3组（n=22）：正常对照组、母子隔离15 min组、母子隔离3 h组。在生后1618 d对3组幼鼠分别行氯化锂-匹罗卡品/杏仁核电刺激诱导的惊厥实验。结果:与正常组比较，P2~P15/17幼鼠每天母子隔离3 h应激组，氯化锂-匹罗卡品诱导惊厥发作的潜伏期明显缩短（P<0.05）；杏仁核点燃惊厥阈值明显降低、完全点燃所需刺激次数明显减少（P<0.05），而P2~P15/17幼鼠每天母子隔离15 min应激组与正常组幼鼠在上述几个方面比较，则差异无统计学意义（P>0.05）。结论:早期母子隔离3 h的应激可通过缩短氯化锂-匹罗卡品诱导惊厥发作的潜伏期、降低杏仁核点燃惊厥阈值、减少完全点燃所需的刺激次数，导致2种幼鼠癫痫模型的易感性增加。

Abstract:

Objective:To explore the influence of early maternal isolation stress on the epilepsy susceptibility of li-pilocapine and amygdala kindling induced seizures models in infantile rats. Methods:Totally 66 newborn SD rat pups were enrolled and randomly divided into three groups (n=22)：the normal control group, the maternal separating 15 minutes group and the maternal separating 3 hours group. All rats were performed with li-pilocapine and amygdala kindling induced-seizures experiment at 16/18 days after the birth. Results: The latency period of li-pilocapine induced-seizures was obviously shortened and the convulsive threshold of amygdala kindling as well as the stimulating number needed for full kindling were markedly decreased in the maternal separating 3 hours group compared with those in the normal control group at 2～15/17 days after the separation (P<0.05). However, there was no significant difference in the above indicators between the normal control group and the maternal separating 15 minutes group at 2～15/17 days after the separation(P>0.05). Conclusion:The stress of early maternal separating for 3 hours can shorten the latency period of li-pilocapine induced-seizures, decrease the threshold of amygdala kindling and the stimulating number needed for full kindling thus enhance the susceptibility of the two seizure models in infantile rats.