高脂高胆固醇饮食致C57BL/6J小鼠肺组织脂质蓄积的研究

High fat/high cholesterol diet induced lipid accumulation in pulmonary tissues of C57BL/6J mice

摘要:

【摘 要】目的：研究高脂高胆固醇饮食（Paigen饮食）是否可以导致C57BL/6J小鼠肺组织的脂质蓄积。方法：66只C57BL/6J雄性6~8周龄小鼠随机分为２组，分别喂饲普通饮食和Paigen饮食，于喂养12、16、20周３个时间点，取主动脉做冰冻切片，油红O染色观察粥样硬化斑块形成情况；肺组织冰冻切片油红O染色，观察肺组织脂质蓄积情况；体外培养A549细胞观察用不同浓度低密度脂蛋白（Low density lipoprotein，LDL）处理后，A549细胞内脂质蓄积情况。结果：Paigen饮食可导致C57BL/6J小鼠主动脉粥样硬化；同时，不同时间点C57BL/6J小鼠肺组织油红O染色结果提示该饮食还可以导致C57BL/6J小鼠肺组织出现脂质蓄积，并随着时间延长而加剧；不同浓度LDL刺激A549细胞的油红O染色结果提示：A549细胞的脂质蓄积与LDL处理的浓度间存在剂量效应关系。结论：Paigen饮食可以导致C57BL/6J小鼠肺脏出现时间依赖性的脂质蓄积，其机制可能与肺泡Ⅱ型上皮细胞的脂质代谢异常有关。

Abstract:

【Abstract】Objective：To investigate whether high fat/high cholesterol diet（Paigen diet） could lead lipid accumulation in pulmonary tissues of C57BL/6J mice. Methods：Sixty six C57BL/6J male mice were randomly divided into two groups and were treated with regu－lar diet and high fat/high cholesterol diet respectively. The pulmonary tissues were collected at the 12th，16th week and the 20th week after feeding respectively. The aortas atherosclerotic plaque and lipidosis in pulmonary tissues were determined by oil red O staining. A549 cells were cultured in vitro and were treated with different concentrations of low density lipoprotein（LDL） to observe the effect of cholesterol loading on alveolar lipid accumulation in vitro. Results：Aorta atherosclerosis plaques were observed in high fat/high cholesterol diet treated C57BL/6J mice. Oil red O staining of pulmonary tissues at different time points suggested that high fat/high cholesterol diet could lead to time-dependent lipid accumulation in pulmonary tissues of C57BL/6J mice. Oil red O staining of A549 cells implied that there was a dose-effect relationship between the lipidosis and the concentrations of LDL. Conclusion：High fat/high cholesterol diet could lead to time-dependent lipid accumulation in pulmonary tissues of C57BL/6J mice，which partly due to the disruption of lipid homeostasis in alveolar type Ⅱ epithelial cells.