MCT1、CD147、CD44 蛋白在非小细胞肺癌中的表达相关性及意义

Correlation and significance of MCT1，CD147and CD44 protein expressions in non-small cell lung cancer

摘要:

目的：探讨单羧酸转运蛋白1（Monocarboxylate transporters 1，MCT1）、CD147、CD44 蛋白在非小细胞肺癌（Non-small cell lung cancer，NSCLC）表达相关性及其在NSCLC发生发展、预后中的意义。方法：取经病理确诊的50例NSCLC患者癌组织（肺癌组）、远癌组织（距肿瘤边缘大于5 cm，远癌组）和21例肺良性病变组织（对照组）。采用免疫组化方法检测 MCT1、CD147、CD44蛋白表达。结果：（1）肺癌组中 MCT1、CD147、CD44蛋白的阳性表达均明显高于远癌组和对照组（P＜0.05）；（2）MCT1在肺癌细胞膜中表达与CD147、CD44蛋白表达（χ2=7.284，P=0.007； χ2=6.522，P=0.011）均存在相关性；（3）MCT1、CD147、CD44蛋白的表达与NSCLC患者的性别、年龄、肿瘤大小、PTNM分期均无关，但与肿瘤的组织类型（ χ2=12.888，P=0.039； χ2=3.997，P=0.043； χ2=3.982，P=0.000； χ2=7.670，P=0.035）、淋巴转移（χ2=13.406，P=0.021； χ2=13.406，P=0.021； χ2=11.809，P=0.039； χ2=12.493，P=0.000）显著相关，另外 MCT1（细胞膜）蛋白表达还与肿瘤分化程度（ χ2=7.135，P=0.013）及吸烟史（ χ2=6.623，P=0.010）显著相关，CD44与肿瘤的分化程度显著相关（χ2=5.486，P=0.000）。结论：在NSCLC患者组织中 MCT1、CD147、CD44蛋白表达均高于癌周组织及肺良性病变组织，统计结果显示3者在NSCLC细胞膜表达具有显著相关性。MCT1、CD147、CD44可能参与肿瘤的发生、发展及远处淋巴结转移，它们可能作为检测NSCLC和预后评价指标。

Abstract:

Objective：To analyze the correlation of monocarboxylate transporters 1（MCT1），CD147 and CD44 protein expressions in non-small cell lung cancer（NSCLC） and to discuss their respective clinico-pathological value in the occurrence，development and prognosis of NSCLC. Methods：Fifty specimens of NSCLC tissues（pulmonary cancer group），peritumoral tissues（more than 5 cm far from the tumor margin，peritumoral group） and 21 specimens of benign pulmonary disease tissues（control group） were enrolled. The expressions of MCT1，CD147 and CD44 protein were detected by immunohistochemistry. Results：（1）The positive expression rates of MCT1，CD147 and CD44 protein in pulmonary cancer group were significantly than those in peritumoral group and control group（P<0.05）；（2）Significant correlations between MCT1 expressions in the cell membrane and expressions of CD147 and CD44 were existed（ χ2=7.284，P=0.007；χ2=6.522，P=0.011）；（3）The expressions of MCT1，CD147 and CD44 protein in NSCLC were not correlated with the age，gender，site，tumor size and pTNM stage，but correlated significantly with the histological type（ χ2=12.888，P=0.039； χ2=3.997，P=0.043； χ2=3.982，P=0.000；χ2=7.670，P=0.035） and lymph node metastasis（ χ2=13.406，P=0.021；χ2=13.406，P=0.021；χ2=11.809，P=0.039； χ2=12.493，P=0.000）. In addition，MCT1 expressions in the cell membrane were correlated significantly with the tumor differentiation degree（χ2=7.135，P=0.013） and smoking（χ2=6.623，P=0.010）. CD44 was correlated significantly with the tumor differentiation degree（χ2=5.486，P=0.000）. Conclusions：The expressions of MCT1，CD147 and CD44 protein in specimens of NSCLC are significantly higher than those in peritumoral tissues and benign pulmonary disease tissues. Statistics shows that significant correlations among expressions of MCT1，CD147 and CD44 protein in NSCLC cell membrane are existed. MCT1，CD147 and CD44 protein may participate in the occurrence，development and the distant lymph node metastasis of cancer，thus can be regarded as the evaluation index for NSCLS.