低频脉冲磁场对兔膝关节软骨细胞白介素-1β、肿瘤坏死因子-α表达与分泌的影响

Effect of low frequency pulsed electromagnetic field on the expression and secretion of IL-1β and TNF-α in rabbit’s knee chondrocytes

摘要:

目的：测定在低频脉冲磁场（Low frequency pulsed electromagnetic field，LFPEMF）刺激的情况下，兔膝关节软骨细胞白介素-1β（Interleukin-1β，IL-1β）与肿瘤坏死因子-α（Tumor necrosis factor-α，TNF-α）表达与分泌的变化。方法：分离培养由兔骨髓干细胞（Marrow stem cells，MSCs）诱导分化形成的软骨细胞，分别使用不同强度的LFPEMF进行电磁辐射刺激，用免疫印迹法测细胞中IL-1β与TNF-α的表达状况，并用ELISA检测细胞培养液中IL-1β与TNF-α的水平。结果：施加LFPEMF的兔膝关节软骨细胞中IL-1β与TNF-α的表达无明显差异，但其培养液中IL-1β与TNF-α的水平有明显降低，并且随着施加脉冲磁场刺激强度的增加，IL-1β与TNF-α的降低程度增强。结论：LFPEMF可以抑制体外兔膝关节软骨细胞IL-1β与TNF-α的分泌。

Abstract:

Objective：To investigate the changes of the expression and secretion of interleukin-1β（IL-1β） and tumor necrosis factor-α（TNF-α） in rabbit’s knee chondrocytes under the stimulation of low frequency pulsed electromagnetic field（LEPEMF）. Methods：Marrow stem cells（MSCs） of rabbits were obtained and differentiated into chondrocytes in vitro. PEMF with different intensities was applied to stimulate the chondrocytes. The expression levels of IL-1β and TNF-α in the chondrocytes were measured by Western blot and their levels in the cultured medium were measured by enzyme linked immunosorbent assay（ELISA）. Result：PEMF did not exert obvious effect on the expression levels of IL-1β and TNF-α in the chondrocytes but it can reduce the secretion levels of of IL-1β and TNF-α in the cultured medium in a dose-dependent way. Conclusion：PEMF can inhibit the secretion of IL-1β and TNF-α in cultured rabbit’s chondrocytes.