三七总皂苷对棕榈酸诱导的HepG2细胞炎症反应的保护作用

Protective effects of Panax Notoginseng Saponins on palmitic acid induced inflammation in HepG2 cells

摘要:

目的：观察三七总皂苷（Panax notoginseng saponins，PNS）对棕榈酸（Palmitic acid，PA）诱导的HepG2细胞炎症反应的作用，探讨PNS在抗炎作用中可能的机制。方法：体外培养HepG2细胞，根据实验要求设置对照组、PA处理组、PNS预保护组。采用MTT实验检测PA和PNS对HepG2细胞增殖的影响；油红O染色检测HepG2胞内脂质沉积；Real time PCR检测HepG2细胞肿瘤坏死因子α（Tumor necrosis factor-α，TNF-α）基因表达水平；ELISA法检测TNF-α释放水平；免疫荧光检测核转录因子（Nuclear factor kappa B，NF-κB）激活。结果：PA处理可诱导HepG2细胞凋亡、脂质沉积及炎症反应；PNS预处理则能够显著抑制PA诱导的HepG2细胞TNF-α表达及释放；PNS预处理组NF-κB激活被显著抑制。结论：PNS对PA诱导的HepG2细胞TNF-α表达及释放具有抑制作用，提示PNS具有减轻肝细胞炎性反应的作用，抑制细胞NF-κB信号途径可能是该保护作用机制之一。

Abstract:

Objective：To observe the protective effects of panax notoginseng saponins（PNS） on palmitic acid（PA） induced inflammation in HepG2 cells and to discuss the possible mechanism of PNS against inflammation. Methods：HepG2 cells were cultured in vitro. The control group，palmitic acid treatment group（PA group） and PNS treatment group were established according to the experimental requirements. The effects of PA and PNS on the proliferation of HepG2 cells were measured with MTT assay. Lipid accumulation was evaluated morphologically by oil red O staining. Real-time PCR was applied to determine the TNF-α mRNA expression. TNF-α in the cell-free culture supernatants was measured with highly specific enzyme-linked immunosorbent assay（ELISA） kits according to the manufacturer’s directions. Nuclear factor kappa B（NF-κB） activation was examined with immunofluorescence technique. Results：Palmitic acid treatment can induce apoptosis，lipidosis and inflammation in HepG2 cells while PNS pretreatment can suppress the TNF-α expression and releasing induced by PA. NF-κB activation induced by PA was also inhibited by PNS pretreatment. Conclusions：PNS has anti-inflammation effect on hepatocyte. The mechanism of PNS protective effect is partially due to the inhibition of NF-κB activation and TNF-α expression.