CUG连接蛋白1基因在前列腺癌的表达及其功能研究

Expression and function of CUG-binding protein 1 gene in prostate carcinoma

摘要:

目的：探讨CUG连接蛋白1（CUG-Binding Protein 1，CUGBP1）基因在前列腺癌的表达及其蛋白功能。方法：用定量RT-PCR法在前列腺癌组织和癌旁组织中检测CUGBP的mRNA水平。Western blot法检测4种前列腺癌细胞株（22RV1，PC-3，DU145，LNCaP clone FGC）中CUGBP1的表达。利用RNA干扰技术检测PC-3细胞在CUGBP1基因沉默后细胞的增殖与凋亡状态。利用定量RT-PCR法确定其下游调控基因的mRNA水平。结果：CUGBP1在PC-3和22RV1细胞株中高表达。CUGBP1的mRNA在前列腺癌组织中较癌旁组织显著增高。CUGBP1基因shRNA导致CUGBP1基因沉默后抑制了PC-3细胞株的增殖并诱导其凋亡；同时，使前列腺癌细胞在细胞周期上发生G1期阻滞。CUGBP1基因被干扰以后，PC-3细胞株中CDKN1A的mRNA水平发生明显上调，同时BCL2、PCNA、MCM2和MCM3基因的mRNA水平发生明显下调。结论：CUGBP1基因和前列腺癌相关。CUGBP1基因可能通过上调/下调下游基因来调节前列腺癌细胞的凋亡，改变细胞的增殖和细胞周期。

Abstract:

Objective：To study on the expression and function of CUG-binding protein 1（CUGBP1） in prostate carcinoma. Methods: The CUGBP1mRNA levels in the prostate carcinoma specimen and the adjacent non-carcinoma tissues were detected by quantitative RT-PCR. The expressions of four kinds prostate carcinoma cell strains（22RV1，PC-3，DU145，LNCaP clone FGC） in CUGBP1 were detected by Western blot. The proliferation and apoptosis of cell line PC-3 after silencing CUGBP1 were examed by RNA interference technique. The mRNA levels of downstream related genes in cell line PC-3 were determined by quantitative RT-PCR. Results：CUGBP1 was highly expressed in PC-3 and 22RV1. The CUGBP1 mRNA level in prostate carcinoma tissues was higher than that in adjacent non-carcinoma tissues. The CUGBP1 gene silencing，which was induced by shRNA lentivirus，suppressed the proliferation and apoptosis of cell line PC-3 and increased the proportion of cells in G1 phase. The mRNA level of CDKN1A in PC-3 cell line was up-regulated while that of BCL2，PCNA，MCM2 and MCM3 was down-regulated after CUGBP1 gene silencing. Conclusion：CUGBP1 gene may regulate the apoptosis，proliferation and cell cycle of prostate carcinoma cells through up-regulating and down-regulating downstream genes.