骨髓间充质干细胞改善高氧致肺纤维化的研究

Improvement of hyperoxia-induced pulmonary fibrosis by bone marrow mesenchymal stem cells

摘要:

目的：探讨骨髓间充质干细胞（Bone marrow mesenchymal stem cells，BM-MSCs）移植对高氧致新生大鼠肺纤维化的影响。方法：将SD雄性大鼠来源的BM-MSCs移植入受体大鼠体内。将受体大鼠分为正常对照组、正常BM-MSCs移植组、高氧对照组、高氧BM-MSCs移植组4组。实验第14天取各组大鼠肺组织行病理学检查和羟脯氨酸含量测定；提取雌性大鼠肺组织DNA，PCR检测性别决定基因Sry基因表达。结果：高氧对照组肺组织结构紊乱，纤维化明显，羟脯氨酸含量较正常对照组明显升高，高氧BM-MSCs移植组病理改变较高氧对照组减轻，羟脯氨酸含量亦明显降低；PCR结果显示，正常BM-MSCs移植组和高氧BM-MSCs移植组均可检测到Sry基因，高氧BM-MSCs移植组较正常BM-MSCs移植组表达增高。结论：体内移植BM-MSCs可减轻高氧致新生大鼠肺纤维化。

Abstract:

Objective：To investigate the effects of bone marrow mesenchymal stem cells（BM-MSCs） on the pulmonary fibrosis in neonatal rats suffering from hyperoxia. Methods：BM-MSCs were isolated from male SD rats and transplanted into receptor rats. The receptor rats were devided into normal control group，normal BM-MSCs group，hyperoxia control group and hyperoxia BM-MSCs group. On the 14th day，the rats were killed and pulmonary tissue samples were taken for pathological examination and hydroxyproline level test. DNA was isolated from female pulmonary tissues and Sry gene expression was detected by reverse-transcription polymerase chain reaction（RT-PCR）. Results：Pathological examination showed significant fibrosis and structural disorder in pulmonary tissue of hyperoxia control group. The hydroxyproline level in pulmonary tissue samples was significantly higher in hyperoxia control group than in normal control group. The thickness of alveolar wall and the content of hydroxyproline in the lung were reduced significantly in hyperoxia BM-MSCs group. The results from PCR indicated that Sry gene was detected in normal BM-MSCs group and hyperoxia BM-MSCs group，however，the Sry gene expression was increased in hyperoxia BM-MSCs group compared with that in normal BM-MSCs group. Conclusion：BM-MSCs could ameliorate pulmonary fibrosis in neonatal rats suffering from hyperoxia.