氯胺酮对脂多糖诱导人脐静脉内皮细胞核因子-κB表达的影响

Effects of Ketamine on lipopolysaccharide-induced nuclear factor-kappaB expression of human umbilical vein endothelial cells

摘要:

目的：初步探讨胺酮对脂多糖（Lipopolysaccharides，LPS）作用下人脐静脉内皮细胞（Human umbilical vein endothelial cells，HUVECs）核因子-κB（Nuclear factor-kappa B，NF-κB）易位活化的影响及氯胺酮的抗炎效应。方法：HUVECs分为4组：C组（空白对照组）、L组（加入1 μg/ml LPS）、KL1组（3 μg/ml的氯胺酮+ 1 μg/ml LPS）、KL2组（30 μg/ml的氯胺酮+ 1 μg/ml LPS）。采用免疫印记法（Western blot）、反转录聚合酶链式反应（Reverse transcription polymerase chain reaction，RT-PCR）和HPIAS 2000图象分析系统，测定NF-κB p65亚基的易位活化表达。结果：KL1组、KL2组能抑制LPS作用下HUVECs内的NF-κB p65易位表达（P<0.05）。结论：LPS可引起人脐静脉内皮细胞核因子-κB的表达，而氯胺酮可以抑制其作用，且其抑制作用可能与剂量呈正相关。氯胺酮的抗炎机制可能是在核因子-κB转录水平上实现的。

Abstract:

Objective：To investigate the effects of Ketamine on lipopolysaccharide（LPS）-induced translocation of nuclear factor-kappaB（NF-κB） in human umbilical vein endothelial cell nuclei and to explore it’s anti-inflammatory action. Methods：Human um－bilical vein endothelial cells（HUVECs） were divided into four groups:group C（blank control group），group L（1 μg/ml LPS was added），group KL1（3 μg/ml ketamine and 1 μg/ml LPS were added） and group KL2（30 μg/ml ketamine and 1 μg/ml LPS were added）. The tanslocation and expressions of NF-κB p65 in endothelial cell nuclei were detected by Western blot，reverse transcription polymerase chain reaction（RT-PCR） and image analysis system of HPIAS 2000. Results：The tanslocation and expression of NF-κB p65 in en－dothelial cell nuclei induced by LPS were reduced in group KL1 and KL2. Conclusions：The tanslocation and expression of NF-κB p65 in endothelial cell nuclei can be induced by LPS and Ketamine can suppress the activation.There are positive correlations be－tween Ketamine concentration and its inhibitory effect. The anti-inflammatory effect of Ketamine may be achieved by the translocation of ?资B.