辛伐他汀对创伤性脑损伤大鼠胶质原纤维酸性蛋白表达的影响

Effects of Simvastatin on glial fibrillary acidic protein expression in rats with traumatic brain injury

摘要:

目的：利用大鼠创伤性脑损伤（Traumatic brain injury，TBI）模型，探讨胶质原纤维酸性蛋白（Glial fibrillary acidic pro－tein，GFAP）在脑组织的表达变化规律及辛伐他汀（Simvastatin，SIM）对其的影响。方法：5周龄SD大鼠54只，随机分3组：假致伤组、对照组、治疗组，每组18只。对照组、治疗组参照Feeney 氏法制造TBI模型。造模前晚及术后每晚治疗组给予SIM 10 mg/kg灌胃；对照组给予等量淀粉灌胃；于伤后3、12、24 h和3、7、14 d处死取脑。采用免疫组化技术，检测大鼠伤侧海马CA3区 GFAP表达变化情况。结果：对照组伤后3 h即见GFAP阳性表达增强，3、7 d，GFAP阳性细胞增加达到高峰（P＜0.01）。治疗组伤后治疗7 d时GFAP的阳性表达较对照组明显下降（P＜0.05），而治疗≤3 d和治疗14 d时，与对照组相比差异无统计学意义（P ＞0.05）。结论：SIM可抑制GFAP在TBI后的过度表达。

Abstract:

Objective：To study the changing rules of glial fibrillary acidic protein（GFAP） expressions in the rat brain after traumatic brain injury（TBI） and to observe the effect of Simvastatin（SIM）on them based on the TBI rat model. Methods：Totally 54 Sprague-Dwalye（SD） rats aged 5 weeks were employed and divided into sham TBI group，control group and treatment group（n=18）. TBI model was established in control group and treatment group by Feeney’s method. The rats in treatment group were fed SIM 10 mg/kg in the evening pre-injury and in every evening post-injury while those in control group were fed the same dose of starch each time. Then the rats were sacrificed and the rat brains were collected at different time points（3，12，24 h and 3，7，14 d post the injury）. The changes of GFAP expressions in cornu ammonis（CA） area were detected with immunohistochemistry. Results：The GFAP positive cell percentage was higher in control group than in sham TBI group at 3 h after the injury. The GFAP positive cell percentage reached the peak at 3 d and 7 d after the injury（P＜0.01）. The positive expression of GFAP was significantly lowered in treatment group than in control group at 7 d after the treatment（P＜0.05）. There was no difference at 1，2，3 d and 14 d after the treatment between con－trol group and treatment group（P >0.05）. Conclusion：SIM treatment could decrease the over-expression of GFAP in rats after TBI.