基底刚度对血管内皮细胞生长与增殖的影响

Effect of stiffness of substrate on vascular endothelial cell growth and proliferation

摘要:

目的：以细胞生长所粘附的胞外基底的力学特性为对象，通过将人脐静脉内皮细胞（Human umbilical vein endothelial cells，HUVECs）接种于不同刚度的基底上，借以阐明基底刚度对其生长和生物学活性的影响。方法：以双丙烯酰胺和丙烯酰胺复合物为原料，构建不同基底刚度基底膜，经灭菌且胶原交联后接种HUVECs，分别进行MTT试验、鬼笔环肽细胞染色以及流式细胞技术检测细胞的生长周期。结果：随着基底刚度的增强，HUVECs增殖的速度明显受到抑制（P<0.01）；且细胞形态和生长周期也出现相应的变化。结论：基底刚度的改变在HUVECs的生长和增殖方面均有较大的影响，这可为进一步解释动脉粥样硬化中血管内皮细胞形态和功能紊乱提供了相应的生物力学依据。

Abstract:

Objective：To clarify the effect of substrate stiffness on the growth and biological activity of human umbilical vein endothe－lial cells（HUVECs） through studying the mechanical properties of substrate for cell growth and inoculating HUVECs to substrates with different stiffness. Methods：Substrates with different stiffness were built by using bisacrylamide and acrylamide compounds. The sub－strates were sterilized and were cross-linked by collagen to inoculate HUVECs. MTT assay，phalloidin cell staining and flow cytometry analysis were performed to detect the cell cycle. Results：With the enhancement of base stiffness，the proliferation of HUVECs was inhibited significantly（P<0.01） and the cell morphology and growth cycle were also changed accordingly. Conclusions：The changes in substrate stiffness have great impact on the growth and proliferation of HUVECs，which gives further biomechanical evidence to ex－plain the atherosclerotic vascular endothelial cell morphology and dysfunction.