手术对喉鳞状细胞癌血清中抗survivin抗体表达的影响及其与p53和c?鄄myc自身抗体联合检测的意义

Effects of surgery on expressions of serum anti-survivin antibody and significance of combined detection of anti-survivin,anti-p53 and anti-c-myc antibody in laryngeal squamous cell carcinoma

摘要:

目的：了解喉鳞状细胞癌（Laryngeal squamous cell carcinoma，LSCC）患者手术前后血清中抗survivin抗体的变化，并分析抗survivin抗体、抗p53抗体和抗c-myc抗体联合检测在LSCC术后监测中的作用。方法：酶联免疫吸附试验（Enzyme-linked immunosorbent assay，ELISA）用于检测血清样本中抗survivin抗体、抗p53抗体和抗c-myc抗体的表达。结果：（1）在接受根治性手术治疗后，抗survivin抗体、抗p53抗体和抗c-myc抗体的表达水平显著下降。（2）抗survivin抗体在LSCC患者外周血清中高表达，特别是有颈淋巴结转移的样本。（3）抗survivin抗体、抗p53抗体和抗c-myc抗体单独检测时均获得了较高的阳性率；3种抗体联合检测的阳性率并不优于抗survivin抗体的阳性率，但优于抗p53抗体和抗c-myc抗体。结论：抗survivin抗体可能是LSCC的一种有价值的生物标记物。抗survivin抗体、抗p53抗体和抗c-myc抗体联合检测在手术治疗效果监测方面有积极意义。

Abstract:

Objective：To exam the serum anti-survivin antibody levels in post-surgery and pre-surgery samples of patients with laryngeal squamous cell carcinoma（LSCC） and to investigate the potential use of anti-survivin，anti-p53，and anti-c-myc antibodies in the monitoring of LSCC postoperatively. Methods：Enzyme-linked immunosorbent assay（ELISA） was used for the detection of serum anti-survivin，anti-p53，and anti-c-myc antibodies. Results：（1）After radical surgical treatment，the anti-survivin，anti-p53，and anti-c-myc antibodies expression levels were decreased significantly. （2）High levels of anti-survivin antibody were detected in the serum sample of patients with LSCC，especially in samples from individuals with cervical lymphatic metastasis.（3）High positive detection rates were obtained through independent testing of anti-survivin，anti-p53 and anti-c-myc antibodies;the combined positive rate of all three antibodies was inferior to that of anti-survivin antibody but superior to that of anti-p53 and anti-c-myc antibodies. Conclusions：This indicates that anti-survivin antibody may be a biomarker of LSCC. Combined testing of three different tumor markers may be valuable for monitoring of the surgery efficacy.