抗原致敏的树突状细胞联合细胞因子诱导的杀伤细胞对肝癌细胞杀伤活性的研究

Killing activity of antigen-pulsed dendritic cells combined with cytokine induced killer cells against hepatocarcinoma cells

摘要:

目的：观察肝癌细胞抗原致敏的树突状细胞（Dendritic cells，DC）与细胞因子诱导的杀伤细胞（Cytokine-induced killer cells，CIK）共培养后的细胞增殖活性、表型的变化及其对肝癌细胞HepG2的杀伤活性。方法：采集健康供者的外周血单个核细胞（Peripheral blood mononuclear cells，PBMNC），常规诱导出DC、CIK，用肝癌细胞HepG2抗原冲击DC（Ag-DC），并将其与CIK共培养（Ag-DC-CIK），观察CIK和Ag-DC-CIK的细胞表型及增殖活性，并以肝癌细胞HepG2作为靶细胞，用MTT法检测CIK和Ag-DC-CIK的杀伤活性。结果：肝癌细胞抗原致敏的DC与CIK细胞共培养后，Ag-DC-CIK细胞群增殖活性明显增强，且高表达CD3+CD8+、CD3+CD56+双阳性细胞，其增殖活性和杀伤活性较单纯的CIK细胞更高（P<0.05）。结论：肿瘤抗原致敏的DC与CIK共培养获得的Ag-DC-CIK增殖活性和对肝癌细胞的杀伤活性显著高于CIK细胞。

Abstract:

Objective：To observe the proliferation activities，phenotype changes and killing activities of liver cell antigen-pulsed den－dritic cells（Ag-DC） cultured with cytokine-induced killer（CIK） cells against HepG2 hepatocarcinoma cells. Methods：Peripheral blood mononuclear cells（PBMNC） were isolated from healthy donors，then DC and CIK cells were induced. The Ag-DC was co-cul－tured with CIK（Ag-DC-CIK） and the phenotype and proliferation of CIK and Ag-DC-CIK cells were observed. Their cytotoxicity ac－tivities against HepG2 hepatocarcinoma cells were detected by MTT assay. Results：The proliferation of Ag-DC-CIK cell group was significantly enhanced and high expressions of double positive cells of CD3+CD8+ and CD3+CD56+ were observed. The proliferation and cytotoxic activity were higher in Ag-DC-CIK cells than in CIK cells（P<0.05）. Conclusion：The proliferation and liver cancer cell-killing activity of Ag-DC-CIK cells which obtained from co-culturing of Ag-DC and CIK cells are significantly higher compared with those of CIK cells.