涎腺肿瘤中半乳糖凝集素-3与Wnt通路蛋白的相关性研究

Correlation between Galectin-3 and Wnt pathway-related protein in salivary gland tumor

摘要:

目的：研究半乳糖凝集素-3（Galectin-3）与Wnt通路相关蛋白β-链蛋白（β-catenin）、细胞周期蛋白D1（Ｃyclin D1）和E-钙黏附蛋白（E-cadherin）在涎腺肿瘤中表达，探讨它们之间的相关性及临床意义。方法：分析本病理研究所收集的涎腺良恶性肿瘤标本，进行HE染色，光镜观察，以涎腺慢性炎症（38例）作为阴性对照，应用免疫组化（S-P法）检测Galectin-3与β-catenin、E-cadherin、Ｃyclin D1在107例涎腺肿瘤中（58例恶性肿瘤，49例良性肿瘤）的表达情况及相关性。结果：在涎腺恶性肿瘤组织中，Galectin-3蛋白的阳性表达率为72.4％（42/58），显著高于在涎腺良性肿瘤组织中的表达率42.9％（21/49）（P<0.05）；涎腺恶性肿瘤组织中β-catenin、Ｃyclin D1蛋白的阳性表达率分别为75.9％（44/58）和81％（47/58），高于在涎腺良性肿瘤组织中的表达率38.8％（19/49）和59.2%（29/49）（P<0.05），差异有统计学意义。Galectin-3与β-catenin两者的蛋白表达呈正相关（r=0.313，P<0.05）。结论：Galectin-3可能与Wnt信号通路密切相关，这为临床针对Wnt通路的抗肿瘤治疗提供了新的思路。

Abstract:

Objective：To study the expression of Galectin-3，Wnt pathway related protein（β-catenin，Cyclin D1 and E-cadherin） in salivary gland tumors and to explore the relationship and the clinical significance among them. Methods：The benign and malignant tumors of salivary gland specimens were observed under HE staining and light microscopy. Immunohistochemical staining（S-P method） was applied to detect the expression and correlation of Galectin-3，β-catenin，E-cadherin and Cyclin D1 in 107 cases of salivary gland tumors（58 cases of malignant tumors and 49 cases of benign tumors） and 38 cases of chronic inflammation of salivary glands were enndled as negative control. Results：The positive rate of Galectin-3 was 72.4%（42/58） in malignant salivary tumor，higher than that in benign salivary tumor（P<0.05）. The positive rates of β-catenin and Cyclin D1 protein were higher in malignant salivary tumor[75.9%（44/58） and 81%（47/58）] than in benign salivary tumor（P<0.05）. There were significant positive correlations be－tween Galectin-3 and β-catenin（r=0.313，P<0.05）. Conclusions：Galectin-3，Wnt pathway related protein β-catenin and Cyclin D1 are correlated with each other，which may provide new sight in the treatment of salivary gland tumor.