艾司西酞普兰对慢性前列腺炎大鼠行为学的影响

Effect of Escitalopram on the ethology of rats with chronic prostatitis

摘要:

目的：建立慢性前列腺炎（Chronic prostatitis，CP）动物模型并观察其行为学变化，探讨CP的发病机制和临床治疗。方法：50只雄性SD大鼠，随机分成6组：正常对照组、模型组45、60、90 d（每组10只）；治疗15 d组、治疗45 d组（每组5只）。模型组和治疗组大鼠均采用免疫佐剂法造模，治疗组于造模第46天开始给予艾司西酞普兰（Escitalopram）10 mg/（kg·d）灌胃，分别持续15 d和45 d。通过开野实验和糖水消耗试验观察各组大鼠的行为学表现；模型组45、60、90 d处死大鼠取材，治疗组用药后15、45 d取材，观察前列腺组织的大体、光镜病理形态学表现。结果：实验大鼠50只全部进入结果分析。90 d模型组的水平运动和垂直运动次数得分及糖水消耗量[（47.40±17.45）、（5.90±2.80）、（53.80±16.29） ml/24 h]与正常对照组[（70.40±10.35）、（12.80±4.69）、（88.21±21.66） ml/24 h]比较明显减少（P=0.017，P=0.038，P=0.048），差异有统计学意义；与正常组对照比较，45、60 d模型组、治疗15 d组及治疗45 d组的水平运动、垂直运动次数和糖水消耗量的差异无统计学意义（P >0.05）。结论：90 d CP模型大鼠表现出抑郁症状，艾司西酞普兰可改善CP大鼠的病理学表现和抑郁行为。

Abstract:

Objective：To explore the pathogenesis and clinical treatment of chronic prostatitis（CP） by establishing chronic prostatitis rat model and observing their ethological changes. Methods：A total of 50 male Sprague Dawley rats were randomly divided into 6 groups：normal control group（n=10），45 d model group（n=10），60 d model group（n=10），90 d model group（n=10），treatment 15 d group（n=5） and treatment 45 d group（n=5）. The CP rat models were made in the latter five groups by intraperitoneal injection of 0.5 ml pertussis diphtheria tetanus（PDT） vaccine and abdominal subcutaneous multiple points injection of 1.0 ml Freund’s complete adjuvant（FCA） and 35 g/L prostate protein purification fluid at the 0 and 30th d. From the 46th d，the rats in treatment 15 d group and the treatment 45 d group were given intragastric injection of saline containing 10 mg/（kg·d） Escitalopram Oxalate tablets for continuous 15 d and 45 d respectively. The ethological change was determined by open-field test and sucrose consumption test；the morphological change of prostatic tissue was observed by light microscopy. Results：All 50 rats were involved in the result analysis. The horizontal movement，vertical movement times of open-field test and sucrose consumption were significantly decreased in 90 d model group than in normal control group[（47.40±17.45），（5.90±2.80） and （53.80±16.29） ml/24 h vs. （70.40±10.35），（12.80±4.69） and （88.21±21.66） ml/24 h] （P=0.017，P=0.038，P=0.048）. There was no significant difference in the above indicators among 45 d model group，60 d model group，treatment 15 d group and treatment 45 d group（P >0.05）. Conclusions：90 d CP model rats exhibits characteristics of depression. Escitalopram can reduce the prostatic inflammatory responses and depressive behaviors of rats with CP.