寒燥对慢性阻塞性肺病模型层粘连蛋白及透明质酸含量的影响

Effect of cold and dry environment on hyaluronan and laminin in the model of chronic obstructive pulmonary disease

摘要:

目的：了解新疆干燥寒冷环境（寒燥）对慢性阻塞性肺病（Chronic obstructive pulmonary disease，COPD）支气管肺泡灌洗液（Bronchial alveolar lavage fluid，BALF）中层粘连蛋白和透明质酸含量的影响，为新疆寒燥环境下COPD特点的揭示提供参考。方法：利用气管滴注弹性蛋白酶结合熏烟的方法建立COPD模型，气管滴注弹性蛋白酶、熏烟结合寒燥环境应激的方法建立COPD寒燥模型，然后利用酶联免疫吸附测定（Enzyme-linked immunoadsordent assay, ELISA）法测定各组大鼠血清中层粘连蛋白含量，BALF中层粘连蛋白和透明质酸含量。结果：血清层粘连蛋白含量COPD寒燥组高于对照组（P﹤0.01），COPD组含量有高于对照组的趋势，但差异无统计学意义；BALF中层粘连蛋白含量COPD寒燥组高于对照组（P﹤0.05），COPD组含量有高于对照组的趋势，但差异无统计学意义；BALF中透明质酸含量COPD寒燥组和COPD组均高于对照组（P﹤0.05），但COPD寒燥组和COPD组比较差异无统计学意义。结论：经过90 d的建模，２组模型肺部表现均符合COPD的病理特征，但其血清及BALF中层粘连蛋白含量则不尽相同，这可能是西北寒燥环境导致COPD方域化发病的可能机理之一。

Abstract:

Objective：To reveal the effect of cold and dry environment on hyaluronan and laminin in the model of chronic obstructive pulmonary disease（COPD） so as to provide references for treating COPD with local peculiarity of Xinjiang（northwest of China）. Methods：COPD model was established by dripping porcine pancreatic elastase（PEE） in the trachea combined with cigarette smoking and model of COPD cold-dryness was also set up by dripping PEE in the trachea，cigarette smoking combined with cold and dry en－viroment. Bronchial alveolar lavage fluid（BALF） and serum were taken to detect contents of hyaluronan and laminin in rat’s BALF and laminin in rat’s serum of each group with enzyme-linked immunoadsordent assay（ELISA）. The data were analyzed statistically with software SPSS. Results：The results showed that laminin content in serum was significantly higher in COPD cold-dryness group than in control group（P<0.01），but the difference was of no statistical significance between COPD group and control group. Laminin content in BALF was significantly higher in COPD cold-dryness group than in control group（P<0.05），but the difference was of no statistical significance between COPD group and control group. Conclusions：The lung performances of COPD model and COPD cold-dryness model are in accordance with the physiological characteristics of COPD，but laminin contents in serum and BALF are differ－ent，which may be one of the pathogenesis of COPD under the cold and dry environment of northwest china.