BMP-2联合温热化疗对SW480中GDF15和TFF3表达的影响

Effect of bone morphogenetic protein-2 combined with hyperthermic chemotherapy on GDF15 and TFF3 expression in SW480

摘要:

目的：探讨骨形态发生蛋白-2（Bone morphogenetic protein-2，BMP-2）联合温热化疗对SW480中GDF15和TFF3表达的影响。方法：BMP-2作用于大肠癌SW480细胞系，置于43 ℃温热化疗30 min，培养6 h。（1）流式细胞法检测SW480细胞的凋亡；（2）Western blot法检测GDF15和TFF3蛋白表达情况；（3）RT-PCR半定量检测GDF15和TFF3 的mRNA表达。结果：BMP-2联合43 ℃温热化疗后SW480细胞凋亡增加，GDF15和TFF3蛋白表达水平下降，GDF15和TFF3 的mRNA表达亦下调。结论：BMP-2联合温热化疗通过抑制大肠癌SW480的GDF15和TFF3蛋白及其相关基因表达，增强抑制SW480的增殖及转移复发的作用；温热疗法联合化疗对GDF15和TFF3表达抑制有协同作用。

Abstract:

Objective：To explore the effect of bone morphogenetic protein-2（BMP-2） combined with hyperthermic chemotherapy on GDF15 and TFF3 expression in SW480. Methods：BMP-2 was acted on SW480 colorectal cancer cell lines，placed in 43 ℃ hyper－thermic chemotherapy for 30 min and cultured for 6 h. （1）Apoptosis of SW480 cells was detected by flow cytometry assay；（2）GDF15 and TFF3 protein expressions were detected by Western blot；（3）GDF15 and TFF3 mRNA expressions were detected by RT-PCR. Results：SW480 cells apoptosis was increased，GDF15 and TFF3 protein levels were decreased after BMP-2 combined with 43 ℃ hyperthermic chemotherapy. GDF15 and TFF3 mRNA expressions were also reduced. Conclusions：BMP-2 combined hyperthermic chemotherapy can inhibit proliferation and metastasis of SW480 by inhibiting GDF15 and TFF3 protein and mRNA expressions. Hyperthermia combined with chemotherapy has synergistic effect on the inhibition of GDF15 and TFF3 expression.