shRNA claudin-1对SW620细胞侵袭转移的影响

Influence of shRNA claudin-1 on invasion and metastasis of SW620 cells

摘要:

目的：本研究通过抑制SW620中claundin-1的表达量来观察claudin-1对细胞粘附和侵袭转移能力的影响。方法： 首先通过shRNA干扰技术抑制SW620中claudin-1的表达量，看其对基底膜几种介质粘附能力的改变，然后再通过Transwell小室侵袭实验及细胞划痕实验来检测其侵袭转移能力的影响。结果：低表达claudin-1后抑制了SW620细胞对4种不同介质的粘附能力，层粘连蛋白、纤维连接蛋白、胶原蛋白Ⅳ、胶原蛋白Ⅰ的粘附抑制率分别为61%、29%、48%、13%；并且SW620-control组的细胞比SW620-shRNA更具有侵袭力，抑制率高达70%（P＜0.001）；而且抑制claudin-1后细胞的迁移能力明显减弱。结论：抑制claudin-1的表达可以抑制SW620的粘附能力和侵袭转移能力。

Abstract:

Objective：To investigate the effect of claudin-1 on the cell adhesion，invasion and metastasis capacity through inhibiting the expression of claudin-1 in SW620 cells. Methods：The expression of claudin-1 in SW620 cell was inhibited through shRNA in－terfering technique to detect its effect on cell adhesion ability. Then Transwell chamber invasion assay and cell scratch test were applied to detect the invasion and metastasis of cells. Results：Low expression of claudin-1 can inhibit adhesion of SW620 cells to laminin，fibronectin，collagen Ⅳand CollagenⅠ，with adhersive inhibition rate of 61%，29%，48%，12% respectively. The cell invasiveness was stronger in SW620-control group than in SW620-shRNA group，with inhibition rate of 70%（P<0.001）. After the inhibition of claudin-1，cell migration was significantly reduced. Conclusion：Inhibiting claudin-1 expression could suppress the adhesive and invasive a－bility of SW620 cells.